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IBA Lifesciences Strep-tag™ Protein Purification Buffer Set, IBA Lifesciences GmbH

Product Code. 12783064 Shop All IBA Lifesciences Products
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Product Code. 12783064 Supplier IBA Lifesciences Supplier No. 21002001

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Description

Strep-Tactin™ purification buffer set

Strep-Tactin™ Buffer Set contains all buffers necessary for purification of Strep-tag™II or Twin-Strep-tag™ fusion proteins with Strep-Tactin™ as well as for subsequent regeneration of the resin. Strep-Tactin™XT Buffer Set comprises the following buffers:
• 10x Buffer W, 100 ml
• 10x Buffer BXT, 25 ml
• 10x Buffer XT-R, 250 ml 10x Buffer W is a 10x concentrated wash buffer for Strep-Tactin™ and Strep-Tactin™XT resins. It removes unbound molecules from the affinity chromatography matrix during Strep-tag™II and Twin-Strep-tag™ fusion protein purification. 10x Buffer E is a 10x concentrated Strep-Tactin™ elution buffer. The contained desthiobiotin is a modified form of biotin that displaces Strep-tag™II and Twin-Strep-tag™ fusion proteins from the modified biotin-binding pocket of Strep-Tactin™ in a competitive manner, resulting in a mild elution. Please note that 10x Buffer E is not applicable for elution from Strep-Tactin™XT resins. 10x Buffer R is a 10x concentrated Strep-Tactin™ regeneration buffer containing HABA. HABA (2-[4-hydroxy-benzeneazo]benzoic acid) displaces desthiobiotin from the modified biotin-binding pocket of Strep-Tactin™ after the elution of Strep-tag™II or Twin-Strep-tag™ fusion proteins. The yellow buffer turns red upon binding of HABA to Strep-Tactin™, clearly indicating the regeneration process and activity status of the column.
Please note that the Strep-Tactin™ Buffer Set is not applicable for Strep-Tactin™XT.

Specifications

Chemical Name or Material Buffer W, Buffer E, Buffer R
For Use With (Application) Purification of recombinant Strep-Tag fusion proteins on immobilized Strep-Tactin
Quantity 1 Kit
Solution Type Protein Purification Buffer

Safety and Handling

compliance-icons
Product Identifier
  • Strep-Tactin purification buffer set
 Signal Word
  • Warning
 Hazard Category
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2
  • Specific target organ toxicity Category 3
  • Specific target organ toxicity after repeated exposure Category 2
  • Acute toxicity Category 4
 Hazard Statement
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.
  • H335-May cause respiratory irritation.
  • H372-Causes damage to organs.
 Precautionary Statement
  • P264-Wash hands thoroughly after handling.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P302+P352-IF ON SKIN: Wash with plenty of water/soap and water.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P332+P313-If skin irritation occurs: Get medical advice/attention.
  • P337+P313-If eye irritation persists: Get medical advice/attention.

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