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IBA Lifesciences

IBA Lifesciences provides high performance research tools for cell and protein isolation based on their proprietary Strep-tag™ technology. Their product portfolio offers solutions for the entire production chain of recombinant proteins, from cloning, transfection, protein expression and purification to detection, immobilization and assays, as well as innovative cell isolation and expansion tools.

IBA's Cell Selection and Expansion

TACS - Traceless Affinity Cell Selection

Fab-TACS™ technology perfectly combines the benefits of positive and negative cell selection:

High purity, yield and viability
Label-free, non-activated cells due to fully reversible binding
From whole blood, buffy coat, PBMCs or murine samples
Affinity chromatographic, magnetic or fluorescent cell separation

TACS - Traceless Affinity Cell Selection

MHC I Streptamer™

Detection and isolation of antigen-specific T-cells with MHC I Streptamers™:

Label-free cell isolation according to antigen specificity
High specificity, sensitivity, and reproducibility
From single cell suspensions (e.g., PBMCs or murine samples)
Affinity chromatographic, magnetic or fluorescent cell separation

Cell Stimulation and Expansion

CD3: CD28 Streptamers™:

Completely reversible reagents
Termination of stimulation at any given point in time
Adjustable CD3: CD28 ratio
Bead-free

Peptides for T-Cell Stimulation:

Purity >85%
Endotoxin-free

Fluorescent Cell Staining

Detection of antigen-specific T-cells with MHC I Streptamers™ and of receptor-specific T-cells with Fab Streptamers™:

Fully reversible
High specificity, sensitivity, and reproducibility
No leftover staining reagents on the cells after FACS sorting
Optimal preservation of cell effector function
Improved viability of cells

Protein Production and Assay

Cloning

More than 150 Twin-Strep-tag™/Strep-tag™II expression vectors:

Variety of Twin-Strep-tag™/Strep-tag™ II combined with His, FLAG or GST
Different N-/C-terminal orientations
Expression hosts: E. coli, mammalia, insect cells, yeast
Easy cloning via StarGate vectors

Mammalian Expression

The MEXi mammalian expression system is optimised for efficient protein expression and Strep-tag™ purification in mammalian cells:

Perfectly matched HEK293E cell line and cell culture media
Transient expression for time and cost efficient protein production
Multiple expression vectors with different features

Protein Purification

Strep-tag™ technology from the original manufacturer:

High affinity in low pM range
Rapid one-step purification with unparalleled protein purity (>95%)
Mild and specific elution
Tolerates broad range of buffer conditions
Suitable for metalloproteins, membrane proteins, low abundant proteins, and protein complexes amongst others

Detection

For Western blot analysis, immunofluorescence, FACS and ELISA.

Two different detection systems are provided:

Strep-Tactin™ and Strep-Tactin™ XT conjugate for Twin-Strep-tag™/Strep-tag™ II proteins
Monoclonal antibodies against Twin-Strep-tag™/Strep-tag™ II

Immobilization

With near covalent binding affinity, – Immobilisation by Strep-Tactin XT: Twin-Strep-tag can be used for screenings, diagnostic assays, protein biochemistry, protein kinetics and interaction studies, surface plasmon resonance (SPR) experiments (Biacore) or bio-layer interferometry (BLI):

High affinity in low pM range enables measurements over a long time
Minimal non-specific binding
Minimal coefficients of variation (cv)
Chip for SPR experiments can be easily regenerated

Surface-Marker Specific Exosome Isolation

Quick and simple isolation of unlabeled exosomes using Fab-TACS™ for exosomes, which offers:

Highly specific and reproducible isolation
Minimal contamination by other EVs
Label-free exosomes with intact biological functions
Source-independent application
Reversible capture and release of exosomes
A quick and simple protocol, without magnetic beads or ultracentrifugation

Surface-marker Specific Exosome Isolation

Immobilization with Near Covalent Binding Affinity

Reversible capture and analysis of Twin-Strep-tag™ fusion proteins for bio-layer interferometry (BLI) or surface plasmon resonance (SPR) experiments:

Indirect immobilization of the ligand prevents it from being subject to inactivation or influences of the immobilization procedure
High specificity and affinity in low pM range of the Twin-Strep-tag™ - Strep-Tactin™ XT interaction
Ligands can be captured directly from complex samples
Strep-Tactin™ XT biosensors/chips can be easily regenerated by an established procedure, whereby further ligand-analyte interactions can be analyzed with the same biosensor/chip

Immobilization with Near Covalent Binding Affinity

Our Perfect Tool for Isolation of Proteins or Complexes

MagStrep 'Type 3' XT Magnetic Beads offer:

High specificity and low background resulting in high protein purity
High binding affinity and capacity for Strep-tag™ II or Twin-Strep-tag™ fusion proteins
Immunoprecipitation (IP) or co-immunoprecipitation (Co-IP) in batch format and/or high throughput screening for protein purification via pull-downs
Suitable for usage with (semi-)automated magnetic bead purification systems (e.g., Thermo Scientific™ KingFisher™) for automated workflows

Our Perfect Tool for Isolation of Proteins or Complexes

Efficient Purification Even of Large Proteins

The Strep-Tactin™ XT 4Flow™ Starter Kit consists of everything you need for a quick start:

Strep-Tactin™ XT 4Flow™ gravity flow column
All buffers required for washing, elution, and regeneration
Strep-Tactin™ conjugated with horseradish peroxidase (HRP; for Western blot detection)
Short protocol

Efficient Purification Even of Large Proteins

Strep-Tactin™ for Reversible Binding of Biotinylated Proteins

Applicable for purification, detection, and immobilization of biotinylated molecules:

Suitable for proximity-dependent biotin identification (BioID) experiments
Mild and highly specific elution facilitating analysis of protein complexes
Strep-Tactin™ Sepharose™ and Strep-Tactin™ Superflow™ for gravity flow column-based affinity purification
MagStrep 'Type 3' Strep-Tactin™ magnetic beads are suitable for batch purification as well as pull-down assays

Strep-Tactin™ for Reversible Binding of Biotinylated Proteins

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IBA Lifesciences Strep-Tactin™ 4Flow™ high capacity 50% suspension

Strep-Tactin™ 4Flow™ high capacity 50% Suspension to purify Strep-tag™II or Twin-Strep-tag™ fusion proteins

Pricing and Availability
Specifications

Particle Size	50 to 150 μm
Ligand Type	Twin-Strep-tag™; Strep-tag™II; cross reactivity: biotin
Stationary Phase	Agarose
Type	Bulk Resin
Target	Proteins tagged with Twin-Strep-tag™; Strep-tag™II; cross reactivity: biotin
Format	Bottle
Description	4% agarose, crosslinked
Final Product Type	Affinity chromatography equipment
Protein Form	Tetrameric protein
Form	Resin Slurry
Binding Capacity	20 mg/mL resin
Column Type	Suspension
Concentration	50% suspension

IBA Lifesciences Strep-Tactin™XT 4Flow™ High Capacity Resin

Strep-Tactin™XT 4Flow™ high capacity resin is a 50% suspension to purify Strep-tag™II or Twin-Strep-tag™ fusion proteins

Pricing and Availability

IBA Lifesciences 10x Buffer BXT

IBA Lifesciences GmbH 10x Buffer BXT; Strep-Tactin™XT Elution B. is a ready-to-use buffer for elution of Strep-tagged proteins from Strep-Tactin™ XT.

Pricing and Availability

IBA Lifesciences MagStrep Strep-Tactin™ XT Beads

MagStrep Strep-Tactin™ XT beads are magnetic beads (5% suspension) to purify Strep-tag™ II or Twin-Strep-tag™ fusion proteins.

Pricing and Availability
Specifications

Particle Size	20 to 40 μm
Type	Magnetic Agarose Bead
Target	Twin-Strep-tag or Strep-tag II
Form	Resin Slurry
Formulation	5% (v/v) Suspension in PBS with 0.1% (w/v) Sodium Azide
Support Type	6% Agarose, Crosslinked

IBA Lifesciences BioLock

BioLock is a Biotin blocking solution for lysates and culture supernatants

Pricing and Availability
Specifications

Type	Blocking Solution

IBA Lifesciences Strep-Tactin™XT 4Flow™ Resin

Strep-Tactin™XT 4Flow™ resin is a 50% suspension to purify Strep-tag™II or Twin-Strep-tag™ fusion proteins

Pricing and Availability

IBA Lifesciences Strep-Tactin™XT 4Flow™ High Capacity Cartridge

Strep-Tactin™XT 4Flow™ high capacity cartridge is a HPLC/FPLC cartridge to purify Strep-tag™II or Twin-Strep-tag™ fusion proteins

Pricing and Availability
Specifications

Particle Size	50 to 150 μm
Target	Twin-Strep-Tag™ and Strep-tag™II
Format	Pre-packed Cartridge
For Use With (Application)	HPLC/FPLC
Support Type	4% agarose, crosslinked

IBA Lifesciences™ Strep-Tactin™ XT Coated Microplate

Ready-to-use 96-well microplate for assay applications

Pricing and Availability

Anhydrotetracycline, IBA Lifesciences

Pricing and Availability

IBA Lifesciences 10x Buffer W - Wash Buffer

Strep-Tactin™ and Strep-Tactin™XT wash buffer

Pricing and Availability

IBA Lifesciences Strep-Tactin™ 4Flow™ Cartridge

Strep-Tactin™ 4Flow™ cartridge is a HPLC/FPLC cartridge to purify Strep-tag™II or Twin-Strep-tag™ fusion proteins

Pricing and Availability
Specifications

Particle Size	50 to 150 μm
Type	High Capacity Chromatography Cartridge
Target	Twin-Strep-Tag™ and Strep-tag™II
Format	Pre-packed Cartridge
For Use With (Application)	HPLC/FPLC
Support Type	4% agarose, crosslinked

IBA Lifesciences Biotin

Pricing and Availability

IBA Lifesciences StrepMAB-Classic

Monoclonal antibody to detect Strep-tag™II and Twin-Strep-tag™ fusion proteins

Pricing and Availability

IBA Lifesciences Strep-Tactin™ Conjugate

Strep-Tactin™ conjugates with AP or HRP for detection of Strep-tag™II and Twin-Strep-tag™ fusion proteins

Pricing and Availability

IBA Lifesciences StrepMAB-Classic Conjugate

StrepMAB-Classic conjugates are monoclonal antibodies with different conjugates to detect Strep-tag™II and Twin-Strep-tag™ fusion proteins

Pricing and Availability
Specifications

Type	Conjugate
Target	Strep-tag™II and Twin-Strep-tag™
Content And Storage	Undiluted: 2°C to 8°C, protect from light; Diluted: -25°C to -15°C

Cell Selection and Expansion

Application Note Central memory T-cells isolated and stained with CD8α and CD62L Fab Streptamer reagents

Filename: ApplicationNote-Central-memory-T-cells-isolated-and-stained-with-CD8α-and-CD62L-Fab-Streptamer-reagents.pdf
Size: 662 KB
Format: application/pdf

Application Note-Combining-MHC-I-and-CD8+-Fab-Streptamer-reagents

Filename: Application_Note_Combining.pdf
Size: 803 KB
Format: application/pdf

Application Note Regulatory T-cells isolated and stained with CD4-CD25 and CD45RA Fab Streptamer reagents

Filename: ApplicationNote-Regulatory-T-cells-isolated-and-stained-with-CD4-CD25-and-CD45RA-Fab-Streptamer-reagents.pdf
Size: 737 KB
Format: application/pdf