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Gibco™ TrypLE™ Select Enzyme (10X), no phenol red
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TrypLE™ Select Enzyme (10X), no phenol red

Brand:  Gibco™ A1217702

617.50 EUR valid until 2024-12-31
Use promo code "21745" to get your promotional price.


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Product Code. 10698118

  • 650.00 € / Each

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For Research Use Only. All usage must comply with product instructions.

This item is not returnable. View return policy

Description

Description

TrypLE™ Select is an animal origin free, recombinant enzyme for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A529, primary human keratinocytes, and embryonic stem cells. We offer a variety of TrypLE™ formulations for a range of cell culture applications.

Compared to trypsin and other dissociation reagents, Gibco™ TrypLE™ Select is:

  • Gentle on cells—maintains cell health for reproducible results
  • Room-temperature stable—convenient to use
  • Easy to use—substitutes directly into existing protocols, with no inactivation required
  • Animal origin free (AOF)—pure, consistent, and effective



    Gentle on cells
    TrypLE™ Select, like trypsin, cleaves peptide bonds on the C-terminal sides of lysine and arginine. However, TrypLE™ Select's exceptional purity increases specificity due to the action of a single enzyme. This reduces damage caused by cleavage from multiple enzymes in trypsin and other extracts.

    Room-temperature stable
    TrypLE™ Select is room-temperature stable and ready to use. TrypLE™ Select cell dissociation reagents remain stable for 24 months at room temperature or 2–8°C, making storage and handling easy and convenient.

    Easy to use
    TrypLE™ Select can be substituted for trypsin in existing protocols. In addition, dilution alone inactivates TrypLE™ Select, avoiding the need for trypsin inhibitors, such as FBS.

    Animal Origin Free (AOF)
    Unlike porcine trypsin and other enzymes, TrypLE™ Select is free of animal-derived components, and is formulated on dedicated animal origin-free equipment.

    Product Intended Use
    For Research Use or Further Manufacturing. Not for use in diagnostic procedures. Customers using Gibco™ TrypLE™ Select in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).

    cGMP Manufacturing and Quality System
    Gibco™ TrypLE™ Select is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

    Concentrated forms of TrypLE™ can be aseptically diluted to 1x using a balanced salt solution containing 1 mM EDTA.
    TRUSTED_SUSTAINABILITY
  • Specifications

    Specifications

    EDTA
    Mammalian Cells
    10 X
    Liquid
    No Phenol Red
    No Calcium, No Magnesium, No Sodium Phosphate
    6.9 to 7.4
    Cell Culture Grade
    Room Temperature Stable
    Animal origin-free
    Storage conditions: 15–30°C. Protect from light.
    Alternate storage conditions: 2–8°C or -5 to -20°C.
    Shipping conditions: Ambient.
    Shelf life: 24 months from date of manufacture.
    Animal Origin-free
    Adherent Cell Culture
    Cell Culture Dissociation Reagent
    Energy efficient, Sustainable packaging
    270 - 320 mOsm/kg
    Gibco™
    500 mL
    Ambient Temperature
    In vitro Bioassay
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    For Research Use or Further Manufacturing. Not for use in diagnostic procedures.