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  5. IBA Lifesciences StarGate pYSG-IBA Vectors for Yeast Expression

IBA Lifesciences StarGate pYSG-IBA Vectors for Yeast Expression
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Vector:
pYSG-IBA 103
pYSG-IBA 105
pYSG-IBA 123
pYSG-IBA 143
pYSG-IBA 145
pYSG-IBA 162
pYSG-IBA 164
pYSG-IBA 167
pYSG-IBA 168
pYSG-IBA 23
pYSG-IBA 25
pYSG-IBA 3
pYSG-IBA 33
pYSG-IBA 43
pYSG-IBA 45
pYSG-IBA 5
pYSG-IBA 62
pYSG-IBA 63
pYSG-IBA 64
pYSG-IBA 65
pYSG-IBA wt1
Förpackningsstorlek
5µg
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Produktkode 15683808

missing translation for 'mfr': IBA Lifesciences 54645001

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Beskrivelse

Designed for high-level expression of recombinant proteins in Yeast by introducing the gene of interest under the control of the Cu++-inducible CUP1 promoter.

pYSG-IBA yeast expression vectors

The pYSG-IBA vector allows the high-level expression of proteins in yeast.

  • The vector carries the copper inducible promoter (CUP1) for controlled high-level expression.
  • The expressed recombinant protein will be localized in the cytoplasm.

 

  • High-level expression in yeast with high copy number
  • Favors correct protein folding and the production of soluble proteins - inclusion bodies rarely form
  • Tightly regulated expression due to Cu++-inducible CUP1 promoter
  • Ampicillin resistance gene and markers Leu2-d and URA3 in every vector
  • Leu2-d auxotrophy marker for selection to increase plasmid copy number during expression
  • URA3 auxotrophy marker for selection after transformation

Please note that cloning into pYSG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pYSG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.

Tekniske data

Format Liquid
For Use With (Equipment) StarGate™ System, Yeast Host Cells
Inducing Agent Copper sulfate
Marker Gene leu2-d and URA3
pH 8
Promoter CUP1
Product Type Acceptor Vector for yeast expression
Storage Buffer 20 μL TE buffer: 10 mm Tris-HCl, 1 mm EDTA / 5 μg
Content And Storage 2°C to 8°C for frequent usage, -20°C for long-term storage
Protein Tag Strep-tag for N-terminal and His Tag (6x) for C-terminal
Concentration 250 ng/μL
Quantity 5 μg
Vector pYSG-IBA 45
Color Clear
For Use With (Application) Cloning Genes of Interest for Use in Functional Expression, Purification, Detection, Immobilization or Separation of Recombinant Proteins
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