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  5. Cytiva rProtein A Sepharose™ Fast Flow Affinity Media

Cytiva rProtein A Sepharose™ Fast Flow Affinity Media

Product Code. p-4591510 Shop All Cytiva Products
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Quantity:
5 mL
25 mL
200 mL
Binding Capacity:
50 mg Human lgG/mL
50 mg Human lgG/mL medium2
Unit Size:
25mL
Each
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Product Code. 10313350

Brand: Cytiva 17127902

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Description

Designed for purification of monoclonal and polyclonal antibodies at labs and process scales

  • Composed of recombinant protein A coupled with cross-linked 4% agarose base matrix
  • High dynamic binding capacity, single-point attachment of protein A, with high recovery, high purity and low ligand leakage
  • No mammalian components involved during manufacturing process
  • Matrix: 4% cross-linked agarose
  • Average particle size: 90μm (d50V)
  • Ligand: 6mg rProtein A/mL medium (E. coli)
  • Binding capacity: ∽50mg human IgG/mL media
  • pH stability working range: 3 to 10
  • Pressure/Flow specification: base matrix 150 to 250cm/hr., 100kPa, XK 50/60 column, bed
TRUSTED_SUSTAINABILITY

Specifications

Quantity 25 mL
Stability Stable in aqueous Buffers commonly used in Protein A Chromatography
For Use With (Application) For PurifiCatIon of Monoclonal and Polyclonal Antibodies at Labs and Process Scales
Storage Requirements 2°C to 8°C
Binding Capacity 50 mg Human lgG/mL
Product Type rProtein A Sepharose Fast Flow Media
Flow Rate 150 to 250 cm/Hoμr
Particle Size 60 to 165 μm
Height (English) Bed 9.84 in.
Height (Metric) Bed 25 cm
Max. Pressure 100 kPa
Matrix 4% Cross-Linked Agarose
pH Range 2 to 9
Storage Buffer 20% Ethanol
Ligand Type Recombinant Protein A (E. coli)
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Safety and Handling

compliance-icons
Product Identifier
  • rProtein A Sepharose
 Signal Word
  • Warning
 Hazard Category
  • Flammable liquid Category 3
 Hazard Statement
  • H226-Flammable liquid and vapour.
 Precautionary Statement
  • P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P405-Store locked up.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contains : ethanol
  • UNKNOWN ACUTE-16.5 per cent of the mixture consists of component(s) of unknown acute toxicity.
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