Cytiva

Cytiva

Welcome to Cytiva Lab Recharge 2022 Program

Offering solutions for all segments and applications in life sciences research, including sample preparation, protein purification, cell culture, filtration and Western blotting.
Shop Cytiva products to advance the discovery and manufacture of therapeutics.
Contact your local Fisher Scientific representative and ask for more information.

Click on the workflow for more tools to support your research:




Protein Research

Choose from Cytiva's portfolio of protein purification tools, from Sephadex™, the chromatography resin that revolutionised protein purification, to the newest magnetic bead-based technology. Cytiva's products support a broad range of chromatography resins and resin methods, including affinity purification, gel filtration, desalting, ion exchange and hydrophobic interaction, all in formats to meet your application requirements.

  • Loose resin up to 500g
  • Chromatography columns such as HiTrap™, SpinTrap™, GraviTrap™ and Tricorn™ columns
  • Pre-packed columns for convenience and efficiency

Western Blotting

Amersham™ Western blotting products deliver reliable protein identification for you time after time: meaningful data, reproducible results.


Key Products

Cell Culture

HyClone™ serum, media buffers and process liquids have helped advance drug discovery and bioproduction processes since 1967, and continue to deliver quality and consistency.

HyClone™ Classical and Serum-Free Media »

Culture cells using our complete line of HyClone™ classical, speciality and transfection media products that support cell growth of CHO, SF9, HEK293, hybridoma, myeloma, insect, NS0 and viral vaccine cell lines.

HyClone™ Fetal Bovine Serum (FBS), Calf Serum, Fetal Bovine Serum Alternatives, and Supplements »

Enhance cell culture performance using a variety of HyClone™ supplements, including Fetal Bovine Serum (FBS), calf serum, engineered serum (FetalClone™ 1, 2, 3) and serum-free supplements (HyClone™ Cell Boost 1-6).

HyClone™ Antibiotics and Reagents »

Separate attachment-dependent cells from substrate or reduce the effect of bacteria, mould or yeast contaminants in your cell culture process.

HyClone™ Stem Cell Products »

Grow stem cells with ease using our specialty line of stem cell products tailored to optimise cell culture performance for a variety of stem cells, including induced pluripotent stem cells (iPSC), mesenchymal stem cells (mSC), human embryonic stem cells (hESC) and epithelial stem cells.

HyClone™ Buffers and Process Liquids »

Manage, control and regulate pH, temperature, osmotic pressure, carbon dioxide and oxygen for your specific cell culture.

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Key Products

DNA and RNA Research

When nucleic acid (DNA or RNA) sample is scarce, amplification can be used to expand the sample quantity for use in multiple experiments. Selecting the most suitable amplification technology will depend on sample type, the molecular biology application where it will be used, and the need to preserve sample for additional testing. Some common technologies for DNA amplification are Phi29 polymerase technology, polymerase chain reaction (PCR), rolling circle amplification (RCA), and isothermal multiple strand displacement amplification (MDA).

Phi29 polymerase technology

Phi29 DNA polymerase-based isothermal DNA amplification is a simple, reliable alternative to other DNA amplification procedures such as polymerase chain reaction (PCR). The highly processive Phi29 DNA polymerase elicits strong strand displacement enabling rapid DNA replication from multiple sites. 

Whole genome amplification

GenomiPhi™ products, powered by Phi29 polymerase technology, are available in kits that provide an easy-to-use method which delivers highly representative and reliable whole genome amplification. Each kit contains all the components necessary for whole-genome amplification by utilising isothermal multiple strand displacement amplification, with minimal hands-on time. Compared with conventional PCR amplification techniques, MDA generates larger sized products with a lower error frequency.

Rolling circle amplification

TempliPhi™, based on Phi29 DNA polymerase, is used to exponentially amplify single- or double-stranded circular DNA by rolling circle amplification (RCA). This isothermal amplification produces microgram quantities of DNA from picograms of starting material in 4 to 6 hours.


Whatman™ Filtration

Filtration is an indispensable step in sample preparation and many analytical methods. Cytiva's Whatman™ products provide the quality, consistency, reliability and convenience to help your lab run smoothly. With a large variety of available sizes, types and specifications, you can be sure to find a filter that fits your application, equipment and workflow.

  • Cellulose, glass and quartz fibre filters
  • Membrane filters
  • Syringe and syringeless filtration devices
  • Capsule filters
  • Sterile and non-sterile options
  • Lab essentials

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Whatman Puradisc syringe filter

  • High performance, dependable single membrane layer filter
  • Available in 11 different membrane types and seven pore sizes to work for samples of many chemical compositions and volumes

Whatman GD/X™ syringe filter

  • Used for heavy, thick, or viscous hard-to-filter samples
  • Multi-gradient glass-fibre layer pre-filters large particles which increases flow rate and volume through the main membrane

Whatman GD/XP syringe filter

  • Used for heavy, thick, or viscous samples with low protein binding and low metals extractables
  • Polypropylene pre-filtration stack provides increased flow rate and volume flow through while eliminating protein binding and inorganic ion extractables that could come from glass fibre pre-filtration 

Whatman SPARTAN™ certified

  • Every batch is tested and certified for HPLC use to ensure batch-to-batch consistency and low levels of UV-absorbing substances
  • Regenerated cellulose membrane exhibits very low protein binding and low levels of extractables in water, methanol and acetonitrile

Whatman Anotop™

  • Recommended for use in ion chromatography 
  • Alumina-based Anopore membrane has very low ion extractables and highly uniform capillary pore structure

Whatman Mini-UniPrep™ (MUP)

  • Designed for HPLC and UHPLC autosamplers to support high throughput testing labs
  • Combines syringe, syringe filter, autosampler vial, cap and septum in a single device for increased efficiency in consumable management

Whatman Mini-UniPrep™ (MUP) G2

  • Designed for HPLC and UHPLC autosamplers to support high throughput testing labs requiring low levels of organic leachables
  • Same design as MUP but a borosilicate glass vial reduces leachables that could come from plastics

Cellulose fibre - quantitative

  • Low ash filters in support gravimetric and other weight-based analyses
  • Available in a variety of thicknesses, retention sizes and surface treatments to support specific applications

Cellulose fibre - qualitative

  • High purity cotton filters support sample separation, clarification and purification in advance of analysis
  • Available in a variety of thicknesses, retention sizes and physical preparations to support specific applications

Glass and quartz fibre

  • The ability to capture very small particles supports environmental analysis and other applications requiring high retention rates
  • Available with a range of particle retention sizes, mechanical strengths and thermal stabilities to support specific applications


Key Products

Syringe Filters

  • Whatman Puradisc
  • Whatman GD/X
  • Whatman GD/XP
  • Whatman SPARTAN certified
  • Whatman Anotop
  • Whatman Mini-UniPrep (MUP) and G2
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Filter papers, and glass/quartz fibres

  • Cellulose fibre – quantitative
  • Cellulose fibre – qualitative
  • Glass and quartz fibre
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Resources

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Products
Cytiva Amersham™ ECL™ Prime Western Blotting Detection Reagent

Cytiva Amersham™ ECL™ Prime Western Blotting Detection Reagent

Enables the use of highly diluted primary and secondary antibodies without reducing sensitivity.  Cytiva Lifescience™ Amersham™ ECL™ Prime Western Blotting Detection Reagent produces high signal intensity and sensitivity. ECL Prime Western Blotting Detection Reagent for 3000 cm2 membrane

Cytiva Amersham™ Protran™ NC Nitrocellulose Membranes: Rolls

Cytiva Amersham™ Protran™ NC Nitrocellulose Membranes: Rolls

Use this versatile nitrocellulose membrane for excellent binding affinity for small proteins and peptides, as well as nucleic acids. Cytiva Amersham™ Protran™ NC Nitrocellulose Membranes: Rolls offer excellent sensitivity, resolution, and low background. X1 Amersham Protran 0.1um Nitrocellulose 150mm x 4m roll

Cytiva Column Holder

Cytiva Column Holder

Holds ÅKTA columns. Cytiva Column Holder for columns with outer diameter between 10 and 50mm. COLUMN HOLDER

Cytiva HiLoad™ Superdex™ 75 pg

Cytiva HiLoad™ Superdex™ 75 pg

For high resolution preparative gel filtration chromatography with short run times and good recovery.  Cytiva Lifescience™ HiLoad™ Superdex™ 75 pg are prepacked XK columns. HILOAD 16/600 SUPERDEX 75 PG

Cytiva Amersham™ Hybond™ P 0.45μm PVDF Membrane

Cytiva Amersham™ Hybond™ P 0.45μm PVDF Membrane

Use for chemiluminescent and fluorescent detection methods for proteins of > Mr 20 000. Cytiva Life Sciences™ Amersham™ Hybond™ P 0.45μm PVDF Membrane is a 0.45 μm pore size hydrophobic membrane, with high physical strength, highly suitable for stripping and reprobing. X10 Amersham Hybond 0.45um PVDF 100mm x 100mm sheets

Cytiva Amersham™ Hybond™ PVDF Membranes: Rolls

Cytiva Amersham™ Hybond™ PVDF Membranes: Rolls

Ideal for Western blotting applications where stripping and reprobing are necessary.  Cytiva Life Sciences™ Amersham™ Hybond™ PVDF Membranes: Rolls  are hydrophobic PVDF membranes. X1 Amersham Hybond SEQ 0.2um PVDF 260mm x 4m roll

Cytiva Disposable PD-10 Desalting Columns

Cytiva Disposable PD-10 Desalting Columns

Use for desalting and buffer exchange, as well as removal of low-molecular weight compounds. Cytiva Life Sciences™ Disposable PD-10 Desalting Columns provide fast, high-recovery. X30 Disposable PD-10 desalting column

Cytiva ExoProStar™ 1-Step

Cytiva ExoProStar™ 1-Step

Enzymatic PCR clean up technology with illustra Exonuclease I and Alkaline Phosphatase combined in one tube to remove unincorporated primers and dNTPs. Cytiva illustra ExoProStar™ 1-Step comes in one tube for maximum convenience. ILLUSTRA EXOPROSTAR-1 STEP (20 RCN)

Cytiva Poly(I)-Poly(C)

Cytiva Poly(I)-Poly(C)

For stimulation of interferon production in vitro.  Cytiva Poly(I)-Poly(C) is supplied as a sterile lyophilised powder. 265 MG POLY (I)-POLY(C) STERILE 265MG STORE AT-20°C Store in Freezer at -20C

Cytiva HyClone™ Phosphate Buffered Saline (PBS)

Cytiva HyClone™ Phosphate Buffered Saline (PBS)

Buffer cell culture growth with PBS PBS 1X .0067M PO4 6X1000ML-CA, MG, Phenol Red, 6x1000 mL

Cytiva Whatman™ 19mm Nuclepore™ Polycarbonate Track-Etched Membranes

Cytiva Whatman™ 19mm Nuclepore™ Polycarbonate Track-Etched Membranes

Manufactured from high-quality polycarbonate film and track-etched for high pore size precision. Whatman™ 19mm Nuclepore™ Polycarbonate Hydrophilic Membranes combine high flow rates and chemical and thermal resistance with sharply defined pores, making them suitable for microscopy, cytology, parasitology, and certain air and oceanography analyses. X100 PC MB 19MM .03UM

Cytiva Heparin Sepharose™ 6 Fast Flow Affinity Chromatography Media

Cytiva Heparin Sepharose™ 6 Fast Flow Affinity Chromatography Media

USe for  separations of biomolecules with an affinity for heparin, including antithrombin III, coagulation factors and other plasma proteins.  Cytiva Life Sciences™ Heparin Sepharose™ 6 Fast Flow Affinity Chromatography Media is fast and reliable and highly suitable for process-scale purification processes. 50 ML HEPARIN SEPHAROSE 6 FAST FLOW 50ML STORE ATambient Store at Room Temperature

Cytiva Amersham™ ECL Select™ Western Blotting Detection Reagent

Cytiva Amersham™ ECL Select™ Western Blotting Detection Reagent

Use as a high sensitivity reagent for chemiluminescent Western blotting detection. Cytiva Life Sciences™ Amersham™ ECL Select™ Western Blotting Detection Reagent  provides highly intense signal output, for detection of medium to very low protein levels. WB DETECTION REAGENT KIT, ECL SELECT, HIGH SENSITIvity chemiluminescent substrate for1000cm2 of

Cytiva Whatman™ Benchkote Plus Surface Protector Sheets

Cytiva Whatman™ Benchkote Plus Surface Protector Sheets

Prevent many common sources of laboratory contamination, such as those involving hazardous materials on shared workspace benches. Whatman™ Benchkote Plus bench liner can also help with the disposal of hazardous materials. X50 SHEET BENCHKOTE+ 50X60 CM

Cytiva Sephadex™ LH-20

Cytiva Sephadex™ LH-20

Use for molecular sizing of natural product such as steroid, terpenoid, lipid, low molecular weight peptide. Cytiva Life Sciences™Sephadex™ LH-20 is a liquid chromatography media useful to analytical and industrial scale for preparation of closely related molecular species. SEPHADEX LH-20, 25 GRS

Cytiva HiTrap™ Protein G HP Prepacked Columns

Cytiva HiTrap™ Protein G HP Prepacked Columns

Use for fast, easy separations of IgG from different species and subclasses. Cytiva Life Sciences™ HiTrap™ Protein G HP Prepacked Columns are prepacked with Protein G Sepharose™. HITRAP PROTEIN G HP, 1 X 1 ML

Cytiva Whatman™ Binder-Free Glass Microfiber Filters, Grade GF/B

Cytiva Whatman™ Binder-Free Glass Microfiber Filters, Grade GF/B

Filters fine particles with high wet-strength and high loading capacity. Whatman™ Binder-Free Microfiber Filters, Grade GF/B are particularly useful for liquid clarification and liquid scintillation counting. X100 FILTER GLASS GF/B 37MM

Cytiva GenomiPhi V2 DNA Amplification Kit

Cytiva GenomiPhi V2 DNA Amplification Kit

Amplify whole genomes with high efficiency and representation. Cytiva Life Sciences™ illustra™ GenomiPhi V2 DNA Amplification Kit provides whole genome amplification with yields of 4 to 7μg. ILLUSTRA GENOMIPHI V2 DNA AMPLIFICATION KIT 100reactions 100reactions Store in freezer at -80C

Cytiva HyClone™ DMEM/F12 1:1 Media

Cytiva HyClone™ DMEM/F12 1:1 Media

Used for culturing many different mammalian cells including MDCK, Glial cells, fibroblasts, and endothelial cells. HyClone™ DMEM/F12 1:1 Media supports cell culture growth and biomanufacturing processes and is manufactured using ISO 9001:2000-certified processes. 1000ML DMEM/F-12 1:1

Cytiva HyClone™ SFM4Insect™ Media

Cytiva HyClone™ SFM4Insect™ Media

Support growth of many key insect cell lines and production of a variety of recombinant proteins. HyClone™ SFM4Insect™ Media is free of animal-derived components (ADCF). SFM4INSECT + L-GLUTAMINE LIQUID 1LL-Glutamine Liquid, Size=1000 mL

Cytiva GSTrap™ 4B Prepacked Columns: 7 × 25mm

Cytiva GSTrap™ 4B Prepacked Columns: 7 × 25mm

Use for convenient, high capacity one-step purification of glutathione S-transferase (GST) tagged proteins. Cytiva Life Sciences™ GSTrap™ 4B Prepacked Columns: 7 × 25mm are prepacked with Glutathione Sepharose™ 4B GSTRAP 4B, 1 X 1 ML

Cytiva HyClone™ McCoy's 5A Media

Cytiva HyClone™ McCoy's 5A Media

Support cell culture growth and biomanufacturing processes with HyClone™ McCoy's 5A Media, manufactured using ISO 9001-certified processes.

500 ML MCCOY'S 5A WITH L-GLUTAMINE, WITHOUT PHENOLRed 500mL Store in 4C Fridge

Cytiva Whatman™ Qualitative Filter Paper: Grade 1 Sheets

Cytiva Whatman™ Qualitative Filter Paper: Grade 1 Sheets

Covers a wide range of laboratory applications and is frequently used for clarifying liquids. Whatman™ Qualitative Filter Paper: Grade 1 Sheets are widely used standard grade filter paper for routine applications with medium retention and flow rates. X500 FILTER PAPER GR. 1 75X100MM

Cytiva Whatman™ Puradisc™ 4mm Sterile Syringe Filters

Cytiva Whatman™ Puradisc™ 4mm Sterile Syringe Filters

Designed for aqueous and organic solutions containing high debris levels. Whatman™ Puradisc DpPP Syringe Filters: Nonsterile tolerates high heat and mechanical stress. X50 SYRINGE FILTERS 4MM PVDF, STERILE 0.2 MICRONPORE SIZE

Cytiva Polyethylene (PE) Capillary Tubing

Cytiva Polyethylene (PE) Capillary Tubing

For use with chromatography applications CAPILLARY TUB PTFE,5M,1.1ID,1.8OD

Cytiva pI Markers

Cytiva pI Markers

Run these markers in parallel with the experimental samples on isoelectric focusing gels. Cytiva Life Sciences™ pI Markers are lyophilized mixtures of stable, salt-free, highly purified proteins. IEF MARKER, BROAD PI CALIBRATION KIT PH 3 TO 1000 Store in Fridge at 4C

Cytiva HyClone Characterized Fetal Bovine Serum (FBS), Australian Origin

Cytiva HyClone Characterized Fetal Bovine Serum (FBS), Australian Origin

Enhance growth of cells with Australia sourced HyClone Characterized Fetal Bovine Serum. 500ML Certified Austrialian (FBS)

Cytiva Glass Plate Divider

Cytiva Glass Plate Divider

Accessory for use with Hoefer SE 600 vertical unit. GLASS PLATE CLUB SANDWICH DIVIDER, NOTCHED FORSE 600 Ruby, SE 600 and SE 660 180mm x 160mm

Cytiva HiTrap™ AVB Sepharose™ Columns

Cytiva HiTrap™ AVB Sepharose™ Columns

Enables purification of adeno associated viruses (AAV) in one step, with high purity and excellent yields. Cytiva HiTrap™ AVB Sepharose™ Columns are pre-packed with affinity chromatography medium (resin) for simple operation with a syringe, pump or chromatographic system. X5 AVB Sepharose high performance 1mL store at 4°C

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ÄKTA™ Quote/ Info Request
GEAkta

ÄKTA™ start protein purification system

You won’t believe how easy it is.

From method creation and sample loading to fraction collection and data analysis, we’ve thought about how to make your work easier at every step. Let ÄKTA start transform your manual purification workflow into a convenient automated solution that delivers reproducible results.

It’s like having an expert with you all the way.

UNICORN start system control software is user-friendly, guiding you through each step in your purification, from creating a protocol and setting up your run to evaluating your results and creating a custom report. See how UNICORN start software saves you time and gives you valuable insight into your run or purification.

ÄKTA start offers the following features:

  • Compact solution for quick and reliable one-step protein purification
  • Sample injection and fraction collection options
  • Quick start methods and templates for common purification techniques
  • Intuitive touchscreen display and real-time monitoring

Specifications:

  • Flow rate: 0.5 to 5mL/min
  • Pump type: peristaltic pump - single channel, four-roller pump head with low pulsation
  • Max. operating pressure: 0.5MPa
  • UV, fixed wavelength: Yes, 280nm
  • Conductivity range: 0 to 300mS/cm
  • Software: UNICORN start 1.0 or higher
Cytiva logo

Cytiva: speed, efficiency, capacity

Cytiva is a global provider of technologies and services that help advance and accelerate the development and manufacture of therapeutics. With a heritage of innovation, we continue to move forward, building on the brands you know and have come to rely on for quality and reliability. Our diverse portfolio includes well-recognised brands such as ÄKTA™, Amersham™, HyClone™, MabSelect™ and Whatman™. Cytiva brings speed, efficiency and capacity to life sciences research and manufacturing, enabling the delivery of transformative medicines
to patients.