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  5. Cytiva rmp Protein A Sepharose™ Fast Flow Affinity Media

Cytiva rmp Protein A Sepharose™ Fast Flow Affinity Media

Product Code. p-4591482 Shop All Cytiva Products
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Quantity:
5 mL
25 mL
200 mL
Unit Size:
200mL
25mL
Each
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Product Code. 11369981

Brand: Cytiva 17513801

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Description

Designed for high-purity separation of monoclonal and polyclonal antibodies

  • Non-mammalian-based media composed of recombinant Protein A coupled with Sepharose 4 Fast Flow base matrix by reductive amination
  • Coupling chemistry that minimizes leakage of protein A ligand
  • Multi-point attachment of rProtein A by chemically-stable amide bonds
  • High capacity and good pressure/flow characteristics provide for high throughput

  • Matrix: 4% cross-linked agarose
  • Average particle size: 90μm (d50V)
  • Ligand: recombinant protein A (E. coli)
  • pH Stability working range: 3 to 10
  • pH Stability cleaning-in-place: 2 to 11
  • Binding capacity/mL chromatography medium: >35mg human IgG/mL
  • Pressure/Flow specification: base matrix 150 to 250cm/hr., 100kPa, XK 50/60 column

For therapeutic applications requiring extremely pure eluate fractions of antibodies with non-exposure of antibodies to human or animal derivatives during purification process.

TRUSTED_SUSTAINABILITY

Specifications

Quantity 5 mL
Stability Stable in aqueous Buffers commonly used in Protein A Chromatography
For Use With (Application) For High-Purity Separation of Monoclonal and Polyclonal Antibodies
Storage Requirements 2°C to 8°C
Binding Capacity >Min. 22 mg Human IgG/mL
Product Type rmp Protein A Sepharose Fast Flow Media
Flow Rate 150 to 250 cm/Hoμr
Particle Size 60 to 165 μm
Height (English) Bed 9.84 in.
Height (Metric) Bed 25 cm
Max. Pressure 100 kPa
Matrix 4% Cross-Linked Agarose
pH Range 3 to 10
Storage Buffer 20% Ethanol
Ligand Type Recombinant Protein A (E. coli)
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Safety and Handling

compliance-icons
Product Identifier
  • rmp Protein A Sepharose FF
 Signal Word
  • Warning
 Hazard Category
  • Flammable liquid Category 3
 Hazard Statement
  • H226-Flammable liquid and vapour.
 Precautionary Statement
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contains : ethanol
  • UNKNOWN ACUTE-16.5 per cent of the mixture consists of component(s) of unknown acute toxicity.
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