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  5. Cytiva nProtein A Sepharose™ 4 Fast Flow Affinity Media

Cytiva nProtein A Sepharose™ 4 Fast Flow Affinity Media

Product Code. 11379931 Shop All Cytiva Products
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Quantity:
5 mL
25 mL
pH Range:
2 to 10
3 to 9
Unit Size:
25mL
5mL
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Product Code. 11379931

Brand: Cytiva 17528004

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Description

Used for purification of monoclonal and polyclonal antibodies. Cytiva Lifescience™ nProtein A Sepharose™ 4 Fast Flow Affinity Media contains native protein A coupled to Sepharose 4 Fast Flow.

  • Low leakage of protein A with nearly twice total IgG binding capacity of Protein A Sepharose CL-4B
  • Used in large-scale FDA-approved processes
  • Manufactured without using animal-derived components
  • Rigidity and high degree of substitution of Sepharose 4 Fast Flow matrix facilitates speedy process of large volumes dilute cell culture fluid
  • High chemical, thermal and mechanical stability
  • Withstands high concentrations of hydrogen bond disrupting agents
TRUSTED_SUSTAINABILITY

Specifications

Quantity 25 mL
Stability No significant change in chromatographic performance after 1 week storage using 8 M Urea, 6 M Gua-HCI, 2% Benzyl Alcohol or 20% Ethanol
For Use With (Application) Purification of Monoclonal and Polyclonal Antibodies at Both Laboratory and Process Scale
Storage Requirements 2°C to 8°C
Binding Capacity >35 mg Human IgG/mL
Product Type nProtein A Sepharose 4 Fast Flow Media
Flow Rate 150 to 250 cm/Hoμr
Particle Size 90 μm
Height (English) Bed 9.84 in.
Height (Metric) Bed 25 cm
Max. Pressure 100 kPa
Matrix 4% Cross-Linked Agarose
pH Range 2 to 10
Storage Buffer 20% Ethanol
Ligand Type Native Protein A (S. aureus)
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Safety and Handling

compliance-icons
Product Identifier
  • nProtein A Sepharose 4 Fast Flow, 25 ml
 Signal Word
  • Warning
 Hazard Category
  • Flammable liquid Category 3
 Hazard Statement
  • H226-Flammable liquid and vapour.
 Precautionary Statement
  • P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contains : ethanol
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