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  6. Cytiva Ni Sepharose™ 6 Fast Flow

Cytiva Ni Sepharose™ 6 Fast Flow

Product Code. 10291210 Shop All Cytiva Products
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Quantity:
5 mL
25 mL
100 mL
500 mL
Unit Size:
100mL
25mL
500mL
5mL
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Product Code. 10291210

Brand: Cytiva 17531801

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This item is not returnable. View return policy

Description

Combines the advantages of Ni2 for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose Fast Flow platform. Cytiva Lifescience™ Ni Sepharose™ 6 Fast Flow is part of a range of IMAC chromatography media for capture and intermediate purification.

Ni Sepharose 6 Fast Flow is a member of the Cytiva range of IMAC chromatography media for capture and intermediate purification.

  • Designed for scaling up purification of histidine-tagged proteins.
  • High protein binding capacity and minimal leakage of Ni2+ ions.
  • As a BioProcess medium it fulfills industrial demands for security of supply, robust performance and regulatory support.
  • Available in convenient prepacked HisPrep and HisTrap column formats.
  • Composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix and pre-charged with Ni2+ ions for purification of histidine tagged proteins.
  • The coupling chemistry and the novel ligand is designed to give minimal ligand and Ni2+ leakage while providing high capacity.
  • Available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
  • As member of the BioProcess media range, Ni Sepharose 6 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.

TRUSTED_SUSTAINABILITY

Specifications

Buffer 0.1M HCl, 0.1M NaOH, 8M Urea
Product Type Ni Sepharose 6 FF chromatography media
Quantity 25 mL
Height (English) 9.84 in.
Height (Metric) 250mm
Flow Rate 600 cm/hr.
Max. Pressure 1 bar
pH Range 3 to 12
Particle Size 90μm
Support Type 6% Cross-linked Agarose
Content And Storage 4° to 30°C
Matrix Ni-Sepharose
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Safety and Handling

compliance-icons
Signal Word
  • Warning
 Hazard Category
  • Flammable liquid Category 3
 Hazard Statement
  • H226-Flammable liquid and vapour.
 Precautionary Statement
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P235-Keep cool.
  • P501-Dispose of contents/container to .
 Supplemental information
  • MIXTURE LIST-Contains : Ethanol, Nickel
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