missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Molecular Biology Reagents and Kits
  4. DNA Vectors
  5. IBA Lifesciences pCSG-IBA Mammalian Expression Vector with Secretion

IBA Lifesciences pCSG-IBA Mammalian Expression Vector with Secretion
GREENER_CHOICE

Product Code. 15603858 Shop All IBA Lifesciences Products
Click to view available options
Vector:
pCSG-IBA102
pCSG-IBA104
pCSG-IBA142
pCSG-IBA144
pCSG-IBAwt2
Protein Tag:
His Tag (6x), Twin-Strep-tag
Twin-Strep-tag
Twin-Strep-tag, His Tag (6x)
Untagged
missing translation for 'unitSize'
5µg
Dieser Artikel kann nicht zurückgegeben werden. Rückgaberichtlinie anzeigen

Artikelnummer. 15603858

missing translation for 'mfr': IBA Lifesciences 55001001

Please to purchase this item. Need a web account? Register with us today!

Dieser Artikel kann nicht zurückgegeben werden. Rückgaberichtlinie anzeigen

Beschreibung

Designed for high-level episomal expression in mammalian hosts under G418 selection. Mammalian Expression Vector with Secretion is a large expression vector with universal features for transient expression as well as for generation of stable cell lines (NeoR).

pCSG-IBA mammalian expression vectors
  • Designed for high-level episomal expression in mammalian hosts under G418 selection containing the following elements:
  • Human cytomegalovirus (CMV) immediate-early promoter for high-level expression in a wide range of mammalian cells
  • Episomal replication through Epstein Barr Virus replication origin (oriP) and nuclear antigen encoded by EBNA-1 in human, primate and canine cells and through SV40 replication origin in cells latently infected with SV40 or that express the SV40 large T antigen (e.g. HEK293T, COS-1, COS-7)
  • Stable cell lines can be selected by the neomycin resistance gene (NeoR).
  • In addition, the human cytomegalovirus (CMV) immediate-early promoter enables a high-level expression in a wide range of mammalian cells.

Please note that cloning into pCSG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. Besides to the direct cloning of the gene of interest into pCSG-IBA vectors with Esp3I, another option via a so-called entry-vector is possible.

Spezifikation

Constitutive or Inducible System Constitutive
Marker Gene Ampicillin
Promoter CMV
Product Type Vector
Selection Agent (Eukaryotic) Geneticin™ (G-418)
Content And Storage Recommended: 2°C to 8°C for frequent usage, -20°C for long-term storage
Protein Tag Untagged
Quantity 5 μg
Vector pCSG-IBAwt2
For Use With (Application) Episomal expression (oriP, EBNA-1); BM40 secretion signal
Berichtigung von Produktinhalten

Bitte geben Sie uns Ihr Feedback zu den Produktinhalten, indem Sie das folgende Formular ausfüllen.

Name des Produkts

Indem Sie auf Absenden klicken, erklären Sie sich damit einverstanden, dass Fisher Scientific sich mit Ihnen in Verbindung setzen kann, um Ihr Feedback in diesem Formular zu bearbeiten. Wir werden Ihre Informationen nicht für andere Zwecke weitergeben. Alle bereitgestellten Kontaktinformationen werden in Übereinstimmung mit unserer Datenschutzrichtlinie aufbewahrt. Datenschutzrichtlinie.

Vielen Dank, dass Sie uns helfen, unsere Website zu verbessern. Ihr Feedback wurde übermittelt