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Gibco™ Keratinocyte SFM (1X) Kit
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Product Code. 12539079
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500 mL
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For Research Use Only. All usage must comply with product instructions.
Product Code. 12539079 Supplier Gibco™ Supplier No. 17005042

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201.40 EUR valid until 2026-06-30
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For Research Use Only. All usage must comply with product instructions.

Description

Contains L-glutamine.

Keratinocyte-SFM is supplied with prequalified human recombinant Epidermal Growth Factor 1-53 (EGF 1-53) and Bovine Pituitary Extract (BPE) in separate packaging. Components are not sold separately.

Note:

A Keratinocyte-SFM Master File has been submitted to the FDA. Permission to cross-reference the Master File may be obtained by contacting Technical Services or your local Sales Representative.

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Specifications

Cell Type Keratinocyte Cells, Epithelial Cells
Classification Serum-free
Concentration 1 X
Form Liquid
Product Type Kit
Serum Level Serum-free
Sterility Sterile-filtered
Sterilization Method Sterile-filtered
With Additives Glutamine, Phenol Red
Quantity 500 mL
Content And Storage • 500 mL Keratinocyte-SFM Basal Medium; store in refrigerator (2–8°C) and protect from light
• 25 mg Bovine Pituitary Extract (BPE); store in freezer (-5 to -20°C) and protect from light
• 2.5 μg EGF, Human Recombinant; store in freezer (-5 to -20°C) and protect from light

Complete Medium: store in refrigerator (2–8°C) and protect from light
Culture Type Mammalian Cell Culture
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Frequently Asked Questions (FAQs)

Are the Keratinocyte SFM basal medium and supplement kit available separately?

The basal medium is not available for purchase individually, and can only be ordered as part of the Keratinocyte SFM kit (catalog # 17005-042). The supplements are available separately: Bovine Pituitary Extract is catalog # 13028-014 and the EGF is catalog # PHG0311.

Which serum-free media can be used to culture mouse keratinocytes?

The Keratinocyte SFM without calcium chloride (Cat. No. 37010-022) is appropriate for the culture of mouse keratinocytes. The medium should be supplemented with calcium chloride at a final concentration of 0.02 mM.
Which serum-free media can be used to culture human keratinocytes?

There are a variety of options for serum-free culture of human keratinocytes. Keratinocyte SFM (Cat. No. 17005-042) is a widely used kit that includes the basal medium, bovine pituitary extract (BPE) and Epidermal Growth Factor (EGF). If you would like a serum-free, defined culture system, the options are the Defined Keratinocyte SFM kit (Cat. No. 10744-019) or Epilife medium (Cat. No. M-EPI-500-CA) supplemented with EDGS (Cat. No. S-012-5). If you would like an animal origin free defined system, Epilife medium (Cat. No. M-EPI-500-CA) with S7 supplement (Cat. No. S-017-5) is an excellent choice.
What is the calcium concentration in Keratinocyte SFM (1X) (Cat. No. 17005042)?

Keratinocyte SFM (1X) (Cat. No. 17005042) includes Keratinocyte-SFM (Cat. No. 10724011) and Keratinocytes Supplements (Cat. No. 37000015), which contains Bovine Pituitary Extract (BPE) EGF, Human Recombinant. The complete media is prepared by adding the supplements to the base media. The Keratinocyte-SFM (Cat. No. 10724011) contains calcium chloride at 12.98 mg/L and D calcium pantothenate at 0.29 mg/L.

For how long can I store Keratinocyte-SFM at 4 degrees C?

The base Keratinocyte-SFM has a shelf life of 12 months when stored at 2 to 8 degrees C, protected from light. Keratinocyte-SFM Supplement has a shelf life of 18 months when stored at -20 to 5 degrees C. The complete media should not be stored for longer than 4 weeks at 4 degrees C

For Research Use Only. Not for use in diagnostic procedures.

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