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Cytiva His Mag Sepharose Excel (10%)

Product Code. 17206886 Shop All Cytiva Products
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Quantity:
100 mL
Unit Size:
100mL
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17206886 100 mL 100mL
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Product Code. 17206886 Supplier Cytiva Supplier No. 29358345

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Description

Designed for small-scale purification and screening of histidine-tagged proteins secreted in eukaryotic cell culture.

His Mag Sepharose Excel are magnetic beads designed for rapid, small-scale purification and screening of his-tagged proteins consisting of an Immobilized Metal Affinity Chromatography (IMAC) resin precharged with nickel ions that are strongly bound to a chelating ligand.

  • Simplify small-scale purification and expression screening of histidine-tagged proteins secreted in eukaryotic cell culture
  • Robust parallel screening with high reproducibility
  • Increase target protein yield and decrease degradation through reduced and simplified sample handling
  • Capture histidine-tagged protein from different sample volumes (low microliter to high milliliter scale)
  • Visible and dense Sepharose based magnetic beads give increased simplicity for handling
  • High density of beads ensures rapid capture by magnetic devices

Small-scale capture, purification, and screening of histidine-tagged proteins that are expressed and secreted into supernatants from eukaryotic cells such as insect cells or Chinese Hamster Ovary (CHO) cells in HyClone HyCell CHO cell culture media.

The base of the matrix is based on Sepharose resin, incorporated with magnetite.

Compatible with MagRack 6 and MagRack Maxi, separation tools for handling the beads in microcentrifuge tubes.

TRUSTED_SUSTAINABILITY

Specifications

Binding Capacity < At least 10 mg (histidine)6-tagged protein/ml sedimented medium
Matrix Highly cross-linked spherical agarose including magnetite
Content And Storage 4°C to 30°C, 20% ethanol, 10% medium slurry
Quantity 100 mL
For Use With (Application) Immunoprecipitation and purification of biotinulated biomolecules
Ligand Type IMAC immobilized with Ni

Safety and Handling

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Product Identifier
  • His Mag Sepharose excel, 100 ml (10%)
 Signal Word
  • Warning
 Hazard Category
  • LONG-TERM AQUATIC HAZARD Chronic 3
  • Flammable liquid Category 3
 Hazard Statement
  • H226-Flammable liquid and vapour.
  • H412-Harmful to aquatic life with long lasting effects.
 Precautionary Statement
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contain: ethanol, nickel
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