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Description
p>Simply add substrate and [α-32P]dCTP. As little as 25ng substrate DNA can be used.
- Ambient temperature-stable reactions with a color check for the labeling of DNA to specific activities of >109dpm/μg
- Long shelf life and simple, fast protocol; labeling reaction complete in 10 min.
- No need to remove unincorporated nucleotides before hybridization
- DNA can be labeled in solution or in the presence of low melting point agarose
Suitable for use with dCTP only.
Specifications
Specifications
| Content And Storage | 2°C to 25°C |
| For Use With (Application) | Labelling of DNA from a variety of sources to a high specific activity for use in Southern and Northern blot hybridizations |
| Includes | Labeling reaction Buffered solution of dATP, dGTP, dTTP, Exonuclease-free Klenow Enzyme and random primers in a dried, stabilized form, control DNA 300ng of lambda Hind III DNA in a dried, stabilized form 1 tube |
| No. of Reactions | 30 |
| Product Type | Rediprime II DNA Labeling System |
Safety and Handling
Product Identifier
- Rediprime II, 30 reactions
- Warning
- Skin corrosion/irritation Category 2
- H315-Causes skin irritation.
- EUH210-Safety data sheet available on request.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P264-Wash hands thoroughly after handling.
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P332+P313-If skin irritation occurs: Get medical advice/attention.
- MIXTURE LIST-Contain: trometamol, 2-mercaptoethanol
- UNKNOWN ACUTE-20 per cent of the mixture consists of component(s) of unknown acute toxicity.
- UNKNOWN AQUATIC-Contains 20% of components with unknown hazards to the aquatic environment.
Product Title
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