Protein Biology

Invitrogen™ NuPAGE™ MOPS SDS Running Buffer (20X)

Made with high-purity reagents and are strictly quality controlled

GE Healthcare Amersham™ ECL™ Prime Western Blotting Detection Reagent

Enables the use of highly diluted primary and secondary antibodies without reducing sensitivity.  GE Healthcare Lifescience™ Amersham™ ECL™ Prime Western Blotting Detection Reagent produces high signal intensity and sensitivity.

GE Healthcare Amersham™ Protran™ NC Nitrocellulose Membranes: Rolls

Use this versatile nitrocellulose membrane for excellent binding affinity for small proteins and peptides, as well as nucleic acids. GE Healthcare Amersham™ Protran™ NC Nitrocellulose Membranes: Rolls offer excellent sensitivity, resolution, and low background.

GE Healthcare Amersham™ Hybond™ PVDF Membranes: Rolls

Ideal for Western blotting applications where stripping and reprobing are necessary.  GE Healthcare Life Sciences™ Amersham™ Hybond™ PVDF Membranes: Rolls  are hydrophobic PVDF membranes.

GE Healthcare Amersham™ Hybond™ P 0.45μm PVDF Membrane

Use for chemiluminescent and fluorescent detection methods for proteins of > Mr 20 000. GE Healthcare Life Sciences™ Amersham™ Hybond™ P 0.45μm PVDF Membrane is a 0.45 μm pore size hydrophobic membrane, with high physical strength, highly suitable for stripping and reprobing.

GE Healthcare Disposable PD-10 Desalting Columns

Use for desalting and buffer exchange, as well as removal of low-molecular weight compounds. GE Healthcare Life Sciences™ Disposable PD-10 Desalting Columns provide fast, high-recovery.

Invitrogen™ eBioscience™ Streptavidin FITC Conjugate

Commonly used in indirect staining protocols to detect biotinylated primary antibodies in flow cytometry. Streptavidin binds to biotin with high affinity.

MP Biomedicals™ DAB Tablets

Tetrahydrochloride

Molecular Probes™ Alexa Fluor™ 647 Azide, Triethylammonium Salt

Alexa Fluor™ 647 azide, triethylammonium salt

Invitrogen™ NuPAGE™ MES SDS Running Buffer (20X)

Made with high-purity reagents and are strictly quality controlled

GE Healthcare Amersham™ ECL Select™ Western Blotting Detection Reagent

Use as a high sensitivity reagent for chemiluminescent Western blotting detection. GE Healthcare Life Sciences™ Amersham™ ECL Select™ Western Blotting Detection Reagent  provides highly intense signal output, for detection of medium to very low protein levels.

Invitrogen™ XCell SureLock™ Mini-Cell and XCell II™ Blot Module

Quickly and easily run and blot Novex minigels

Thermo Scientific™ Pierce™ Hydroxylamine-HCl

NH2OH (CAS 7803-49-8) to cleave Asn-Gly peptide bonds or deprotect sulfhydryls following S-acetylthioacetate modification with SATA reagent.

Thermo Scientific™ Sulfo-NHS (N-hydroxysulfosuccinimide), No-Weigh™ Format

Prepare amine-reactive sulfo-NHS-ester crosslinkers or enhance carbodiimide (EDC) conjugation for peptides or proteins using N-hydroxysulfosuccinimide, CAS 106627-54-7.

Invitrogen™ Mini Blot Module

Wet transfer device used exclusively with the Bolt Minigel Tank

Merck Millipore Albumin Fraction V

Derived from bovine serum and packaged in glass bottles for use in biochemistry.

GE Healthcare HiTrap™ Protein G HP Prepacked Columns

Use for fast, easy separations of IgG from different species and subclasses. GE Healthcare Life Sciences™ HiTrap™ Protein G HP Prepacked Columns are prepacked with Protein G Sepharose™.

GE Healthcare Sephadex™ LH-20

Use for molecular sizing of natural product such as steroid, terpenoid, lipid, low molecular weight peptide. GE Healthcare Life Sciences™Sephadex™ LH-20 is a liquid chromatography media useful to analytical and industrial scale for preparation of closely related molecular species.

Thermo Scientific™ TNBSA Solution (2,4,6-trinitrobenzene sulfonic acid) (5% w/v)

Label primary amines (peptides or amino acids) for colorimetric detection (Amax 335nm) using this 5% solution of trinitrobenzene sulfonic acid in methanol.

Thermo Scientific™ Halt™ Protease and Phosphatase Inhibitor Cocktail (100X)

Prevent protein degradation and stabilize phosphorylation with this all-in-one cocktail of protease and phosphatase inhibitors.

Thermo Scientific™ NAb™ Protein G Spin Kit, 0.2 mL

Affinity purify antibodies (IgG) with immobilized recombinant Protein G agarose offered in bottles, spin columns, filter plates and complete purification kits.

Invitrogen™ ABTS, solution

Suitable for use in immunoassays

Thermo Scientific™ Zeba™ Spin Desalting Columns, 7K MWCO, 0.5 mL

Effectively remove salt and other molecules (7K MWCO) from samples and attain excellent protein recovery with these centrifuge-based chromatography columns.

GE Healthcare GSTrap™ 4B Prepacked Columns: 7 × 25mm

Use for convenient, high capacity one-step purification of glutathione S-transferase (GST) tagged proteins. GE Healthcare Life Sciences™ GSTrap™ 4B Prepacked Columns: 7 × 25mm are prepacked with Glutathione Sepharose™ 4B

enQuireBio™ Recombinant Human PRRX1 Protein

A cDNA sequence encoding the PRRX1 was constructed and used to recombinantly synthesize the protein.

Thermo Scientific™ Amino Acid Standard H

Calibrate HPLC analysis of protein hydrolysates using this calibration standard mixture of 18 amino acids, each at 2.5 micromoles/mL in 0.1N HCl.

Invitrogen™ NuPAGE™ Antioxidant

Proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis

Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotin

Biotinylate protein amines (e.g., lysines) with this very popular mid-length (22.4A), water-soluble, NHS-ester biotinylation reagent for antibody or cell-surface labeling.

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