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  6. Thermo Scientific™ Halt™ Protease Inhibitor Cocktail (100X)

Thermo Scientific™ Halt™ Protease Inhibitor Cocktail (100X)

Product Code. 10516495 Shop All Thermo Scientific Products
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Product Code. 10516495 Supplier Thermo Scientific™ Supplier No. 87786

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Description

Inhibit proteolytic degradation during cell lysis and protein extraction with these ready-to-use concentrated stock solutions of protease inhibitors.

Thermo Scientific™ Halt Protease Inhibitor Cocktails are ready-to-use concentrated stock solutions of protease inhibitors for addition to samples to prevent proteolytic degradation during cell lysis and protein extraction procedures.

Halt Protease Inhibitor Cocktails are 100X solutions containing optimized concentrations of six broad-spectrum protease inhibitors stabilized in high-quality dimethylsulfoxide (DMSO). The cocktails effectively inhibit serine-proteases, cysteine-proteases, aspartic acid-proteases and aminopeptidases that are typically present in cellular lysate samples. The identity and concentration of each component is fully disclosed, leaving no uncertainties about variables and compatibility with experiments. Regular and EDTA-free formulations provide for inhibition of metalloproteases and compatibility with 2D electrophoresis, respectively. The stable, concentrated liquid cocktails are easy to use, and the several different package sizes make Halt Protease Inhibitor Cocktails exceptionally convenient for either small-scale or large-scale uses.

Highlights:

  • Better than tablets – the refrigerator-stable, 100X liquid format is more effective and easier to use than individual inhibitors or other formats; just pipette exactly the amount you need
  • No proprietary ingredients – fully disclosed formulation contains AEBSF, aprotinin, bestatin, E-64, leupeptin and pepstatin A in DMSO
  • Multiple package sizes – choose among several package sizes, including single-use microtubes that keep protease inhibitors fresh and reduce the risk of reagent contamination
  • Two popular formulations – regular formulation includes a separate vial of EDTA solution for optional metalloprotease inhibition; EDTA-free formulation ensures compatibility with isoelectric focusing
  • Lysis buffer compatible – use with Thermo Scientific Pierce™ Cell Lysis Buffers or nearly any other commercial or homemade detergent-based lysis reagent

Includes:

Protease inhibitor cocktails with EDTA include separate 0.5M EDTA solution.

TRUSTED_SUSTAINABILITY

Specifications

Product Type Protease Inhibitor Cocktail
Content And Storage Upon receipt store at 4°C
Form Liquid
Product Line Halt
Buffer Lysis Buffer
Reagent Type Protease Inhibitor
Inhibitors Protease Inhibitor
Quantity 1 mL

Frequently Asked Questions (FAQs)

What are the compositions of the Halt Protease Inhibitor Cocktail, EDTA-Free (100X) (Cat. No. 87785) and Halt Protease Inhibitor Cocktail (100X) (Cat. No. 87786)?

Both formulations are the same and have the following composition:

  • AEBSF – 100mM
  • Aprotinin – 80µM
  • Bestatin – 5mM
  • E64 – 1.5mM
  • Leupeptin – 2mM
  • Pepstatin A – 1mM
What is the shelf life of Halt Protease Inhibitor Cocktail (100X)?

When stored as recommended at 4 degrees C, Halt Protease Inhibitor Cocktail (100X) is guaranteed stable for 1 year from the date of receipt.
Can I use just part of the Pierce Protease Inhibitor XL Capsules, EDTA-Free and save part of the capsule for later use?

We do not recommend “splitting” the capsule into smaller portions. Researchers using less than 500 mL should choose a different format (liquid or tablet) of protease inhibitors.
What formats of protease inhibitors do you offer?

We offer standalone protease inhibitors (AEBSF, Aprotinin, PMSF, EDTA, etc.), as well as cocktails or combinations of protease inhibitors in liquid, tablet, and larger capsule formats. These cocktails of protease inhibitors can also be purchased in combination with a cocktail of phosphatase inhibitors, in both liquid and tablet form, for even greater convenience.
What protease inhibtors are present in the Halt Protease Inhibitor Single-Use Cocktail?

The inhibitors included in the Halt Protease Inhibitor Single-Use Cocktail (Cat. No. 78430) are: AEBSF, Aprotinin, Bestatin, E64, Leupeptin, Pepstatin A, and EDTA.
Does the Halt Protease Inhibitor Single-Use Cocktail contain phosphatase inhibitors?

No, the Halt Protease Inhibitor Single-Use Cocktail is a broad-spectrum protease inhibitor that does not contain phosphatase inhibitors.

Safety and Handling

compliance-icons
Product Identifier
  • Halt Protease Inhibitor Cocktail(100X)
 Signal Word
  • Warning
 Hazard Category
  • Skin corrosion/irritation Category 2
  • Serious eye damage/eye irritation Category 2
 Hazard Statement
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.
 Precautionary Statement
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P264-Wash thoroughly after handling.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
 Supplemental information
  • MIXTURE LIST-Contain: Benzenesulfonylfluoride, 4-(2-aminoethyl)-hydrochloride (1:1)

For Research Use Only. Not for use in diagnostic procedures.

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