Biological Buffers

Thermo Scientific™ 6X DNA Loading Dye

Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X DNA Loading Dye. X5 6X DNA LOADING DYE, COMPOSITION: 10MM TRIS-HCL(pH7.6), 0.03% bromophenol blue, 0.03% xylene

Invitrogen™ AccuPrime™ Pfx SuperMix

High-fidelity, high-specificity amplification of DNA fragments 200 RXN PFX SUPERMIX 200 REACTIONS STORE AT -20C

Invitrogen™ Novex™ NuPAGE™ LDS Sample Buffer (4X)

LDS SAMPLE BUFFER (4X), NUPAGE(R) 250ML STORE AT2-8C

Invitrogen™ EDTA (0.5M), pH 8.0

Ready-to-use, certified RNase-free 500 ML 0.5 M EDTA, PH 8.0 500ML STORE AT ROOMtemperature

Thermo Scientific™ Pierce™ StartingBlock™ Blocking Buffers

Block membranes, plates and other samples with these ready-to-use PBS or TBS formulations of a special protein blocker for exceptional nonspecific blocking and broad compatibility. 1 LT BLOCKING BUFFER IN TBS, STARTINGBLOCK T20 1LStore in fridge at 4C

Invitrogen™ Novex™ NuPAGE™ MOPS SDS Running Buffer (20X)

Made with high-purity reagents and are strictly quality controlled NUPAGE MOPS 20XRUN BUF

Invitrogen™ Novex™ 20X Bolt™ MOPS SDS Running Buffer

Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats BOLT MOPS SDS RNBUF 20X

Laemmli SDS Sample Buffer, reducing, 6X

For protein sample preparation to be used in the Laemmli SDS-PAGE system 50ML Laemmli SDS sample buffer, reducing (6X) 50m

Thermo Scientific™ Phire Buffers

Choose from three formats of these buffers to optimize PCR using Thermo Scientific™ Phire™ Hot Start II DNA Polymerase. DETERGENT-FREE PHIRE BUF 6ML

Invitrogen™ AccuPrime™ Pfx DNA Polymerase

Provides higher fidelity than Pfu DNA polymerase 200 RXN PFX DNA POLYMERASE ACCUPRIME(TM) 200reactions Store at -20C

Invitrogen™ Novex™ NuPAGE™ Tris-Acetate SDS Buffer Kit (for Tris-Acetate Gels)

Provide separation of large molecular weight proteins when used with NuPAGE Tris-Acetate SDS Running Buffer TRIS-ACETATE BUFFER KIT, NUPAGE(R) 00

Invitrogen™ Novex™ NuPAGE™ MES SDS Running Buffer (20X)

Made with high-purity reagents and are strictly quality controlled NUPAGE MES 20X RUN BUF

Thermo Scientific™ Phusion™ Buffers

Buffer packs for use in PCR with Phusion High-Fidelity DNA Polymerases X4 Phusion Green High Fidelity buffer pack 1.5ML

Invitrogen™ PBS Phosphate-Buffered Saline (10X) pH 7.4

Dilute to desired concentration 10X PBS BUFFER, PH 7.4 1000 MLsolution, 1000 ml

Invitrogen™ Novex™ NuPAGE™ Antioxidant

Proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis 15ML NUPAGE ANTIOXIDANT

Invitrogen™ RT-PCR Grade Water, Not DEPC-treated

RT-PCR GRADE WATER 10 X 1.7MLAmbion® RT-PCR grade water is tested for

Invitrogen™ Proteinase K Solution (20mg/mL)

General digestion of proteins PROTEINASE K (20MG/ML)5X1.25ML5 tubes

Normal Goat Serum Control, Invitrogen™

Goat Control 2 ML NORMAL GOAT SERUM

Invitrogen™ Novex™ NuPAGE™ Sample Reducing Agent (10X)

Ready-to-use reagent to reduce protein samples for protein gel electrophoresis 250 UL SAMPLE REDUCING AGENT (10X), LIQUID NUPAGE(r) 250µL store at 2° to 8°C

Thermo Scientific™ Phusion Buffers

Choose between two buffer packs to optimize high-fidelity PCR or difficult or long template amplification using Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerases. DETERGENT-FREE HF BUFFER 6 ML

Thermo Scientific™ Restore™ Western Blot Stripping Buffer

Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed. 5 LT RESTORE(TM) WESTERN BLOT STRIPPING BUFFER 5L

Invitrogen™ Acid-Phenol:Chloroform, pH 4.5 (with IAA, 125:24:1)

Premixed, safer, and easier alternative to preparing crystalline phenol solutions 400 ML ACID-PHENOL:CHLOROFORM, PH 4.5 (WITH IAA,125:24:1) 400mL Store at 4°C

Invitrogen™ GlycoBlue™ Coprecipitant (15mg/mL)

Blue dye increases pellet visibility 15 MG GLYCOBLUE 15MG STORE AT -20°C

Invitrogen™ Nuclease-Free Water (not DEPC-Treated)

NUCLEASE-FREE WATER 100 MLAmbion® Nuclease-free water has not been treated

Invitrogen™ Novex™ Tris-Glycine SDS Running Buffer (10X)

Formulated for separation of proteins, in their denatured state, on Novex Tris-Glycine gels TRISGLYSDS RUN BUF(10X)500ML

Thermo Scientific™ SuperBlock™ Blocking Buffers

Block membranes, plates and tissue samples with these optimized solutions containing a protein that provides fast and effective blocking for Western blot, ELISA and IHC. 5 LT SUPERBLOCK(R) BLOCKING BUFFER IN PBS 5LStore in Fridge at 4C

Invitrogen™ Glycogen (5 mg/ml)

Enhances precipitation of nucleic acids GLYCOGEN 5 X 1 MLAmbion® Glycogen is a branched chain carbohydrate

Invitrogen™ UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v)

Nucleaic acid purfication PHENOL/CHLOROFORM/ALCOHOL

Thermo Scientific™ Blocker™ Casein Blocking Buffers

Block membranes, plates and other samples with these ready-to-use PBS or TBS solutions of purified casein for Western blot, ELISA and other detection methods. BLOCKER CASEIN IN PBS 1L4C Ship at Ambient temperature

Invitrogen™ Platinum™ PCR SuperMix High Fidelity

Built-in hot start 5000 RXN PCR SUPERMIX 21 HIGH FIDELITY PLATINUM(R)5000 reactions Store at -20C

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