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Thermo Scientific™ RNase A, DNase and protease-free (10 mg/mL)
Specifically degrade single-stranded RNA at C and U residues with the endoribonuclease RNase A.
Invitrogen™ Lipofectamine™ 2000 Transfection Reagent
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
Versatile transfection reagent that has been shown to effectively transfect the widest variety of adherent and suspension cell lines
Thermo Scientific™ EcoRI (10 U/μL)
The EcoRI restriction enzyme recognizes G^AATTC sites and cuts best at 37°C in its own unique buffer.
| Concentration | 10 U/μL |
|---|
Thermo Scientific™ T4 DNA Ligase, HC (30 U/μL)
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.
Thermo Scientific™ CloneJET PCR Cloning Kit
Efficiently clone PCR products generated with any thermostable DNA polymerase using Thermo Scientific™ CloneJET™ PCR Cloning Kit, a positive selection system.
| Antibiotic Resistance Bacterial | Ampicillin (AmpR) |
|---|---|
| Format | Kit |
| Sample Type | PCR Products |
| Cloning Method | Restriction Enzyme⁄ MCS, Blunt PCR |
| For Use With (Application) | PCR Cloning |
| Vector | pJET1.2/blunt |
| Product Line | CloneJET |
Applied Biosystems™ TaqMan™ Fast Virus 1-Step Master Mix for qPCR
TaqMan Fast Virus 1-Step Master Mix is designed for fast, highly sensitive real-time RT-PCR, even in the presence of challenging PCR inhibitors.
| Shipping Condition | Dry Ice |
|---|---|
| Reverse Transcriptase | M-MLV |
| Form | Liquid |
| InhibitorToleranceSimple | High |
| For Use With (Application) | Microbial Detection,Gene Expression,DNA Quantitation,Presence/Absence |
| PCR Method | 1-step RT-qPCR |
| Multiplex Capability | Up to 3-plex |
| Detection Method | Primer-probe |
| Thermostability | Not Applicable |
| Polymerase | AmpliTaq Fast DNA Polymerase |
| Format | Tube |
| Product Type | 1-Step Master Mix |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudio 12k Flex, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio 7 Flex, StepOne, StepOnePlus, ViiA 7 System |
| Sample Type | Total RNA, Viral DNA, Viral RNA, mRNA |
| Reaction Speed | Fast or Standard |
| Target Specificity | RNA only, Both RNA and DNA |
| Hot Start | Built-In Hot Start |
| Product Line | TaqMan |
Invitrogen™ Qubit™ microRNA Assay Kits
Achieve accurate miRNA quantitation, even in the presence of rRNA, large mRNA, or contaminants, with the Qubit microRNA Assay Kit, which offers 100 and 500 assay formats.
| Excitation/Emission | 498/518 |
|---|---|
| Shipping Condition | Room Temperature |
| Product Type | microRNA Assay Kit |
| For Use With (Equipment) | Qubit Fluorometer |
| For Use With (Application) | microRNA Quantitation |
| Product Line | Qubit |
| Detection Method | Fluorescence |
Invitrogen™ Dynabeads™ Streptavidin Magnetic Beads
Dynabeads Streptavidin Magnetic Beads are designed for high-specificity, low-background capture of target molecules with biotinylated affinity binders such as antibodies, antigens, or oligonucleotides. Available in various sizes and surface chemistries, these beads can be tailored to provide the best fit for specific application needs.
| Shipping Condition | Ambient Temperature |
|---|---|
| Regulatory Status | For Research Use Only |
| Format | Beads in Suspension |
| Bead Type | Magnetic polystyrene-based beads covalently coupled with recombinant Streptavidin |
| For Use With (Equipment) | KingFisher™ Sample Purification System, DynaMag™ magnets |
| Isolation Technology | Magnetic Bead |
| Certifications/Compliance | ISO9001 and ISO13485 |
| Concentration | 10 mg/mL |
| High-throughput Compatibility | High-throughput Compatible |
| Product Line | Dynabeads |
Thermo Scientific™ FastAP Thermosensitive Alkaline Phosphatase (1 U/μL)
Catalyze the removal of 5'- and 3'-phosphate groups from DNA, RNA, nucleotides, and proteins using this thermosensitive alkaline phosphatase .
| Product Type | Alkaline Phosphatase |
|---|---|
| Enzyme | Phosphatase |
| Compatible Buffer | PCR Buffer, Enzyme Buffer |
| Concentration | 1 U/μL |
Thermo Scientific™ T4 DNA Ligase (5 U/μL)
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.
| Product Type | T4 DNA Ligase |
|---|---|
| Enzyme | DNA Ligase |
| Compatible Buffer | 10X T4 DNA Ligase Buffer |
| Concentration | 5 U/μL |
Thermo Scientific™ FastDigest NcoI
The FastDigest NcoI restriction enzyme recognizes C^CATGG sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: Bsp19I).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | Nco I |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
| Overhang | 5' protruding ends |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Inactivation Temperature | 65°C |
| Product Line | FastDigest |
Invitrogen™ Quant-iT™ dsDNA Assay Kits, high sensitivity (HS) and broad range (BR)
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
For highly selective quantitation of double-stranded DNA over RNA, the Quant-iT dsDNA Assay Kits, high sensitivity and broad range, produce fluorescence signals that are linear in the ranges of 0.2-100 ng and 2-1000 ng of DNA, respectively.
| Shipping Condition | Room Temperature |
|---|---|
| No. of Reactions | 1000 Assays (200 μL assay volume) |
| For Use With (Equipment) | Microplate Reader |
| Quantity | 1 kit |
| Product Line | Quant-iT |
| Detection Method | Fluorescence |
Thermo Scientific™ AarI (2 U/μL)
Cut at CACCTGC(4/8)^ sites with AarI restriction enzyme, which performs best at 37°C in its own unique (+oligo) buffer.
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | AarI |
| Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | Unique Buffer (10X Buffer AarI) |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 2 U/μL |