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Invitrogen™ SuperScript™ III Reverse Transcriptase
Invitrogen SuperScript III Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) created by the introduction of several mutations for reduced RNase H activity, increased half-life, and improved thermal stability.
| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Reverse Transcriptase | SuperScript III |
| Final Product Type | First-Strand cDNA |
| Concentration | 200 U/μL |
| Format | Stand-alone Enzyme |
| Size (Final Product) | Up to 12.3 kb |
| Reaction Format | Separate components |
| Reaction Speed | 30 to 50 min. |
| Reagent Type | Reverse Transcription |
| Ribonuclease H Activity | Reduced |
| Optimal Reaction Temperature | 50°C |
| Technique | Reverse Transcription |
| Starting Material | RNA |
Invitrogen™ SuperScript™ IV First-Strand Synthesis System
The SuperScript IV First-Strand Synthesis System for RT-PCR, available with or without ezDNase, is optimized for synthesis of first-strand cDNA from purified poly(A)+ or total RNA.
| GC-Rich PCR Performance | High |
|---|---|
| Format | Kit |
| Reverse Transcriptase | SuperScript IV |
| Size (Final Product) | Up to 12.3 kb |
| Reaction Format | Separate components |
| Final Product Type | First-Strand cDNA |
| Reaction Speed | 10 min. |
| Reagent Type | Reverse Transcription |
| Quantity | Each |
| Optimal Reaction Temperature | 50°C |
| Technique | Reverse Transcription |
| Starting Material | RNA |
Thermo Scientific™ T4 DNA Ligase, HC (30 U/μL)
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.
Thermo Scientific™ Phusion High-Fidelity PCR Master Mix with HF or GC Buffer
Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Phusion High-Fidelity DNA Polymerase |
| Fidelity (vs. Taq) | 52X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | SuperMix or Master Mix |
| Product Type | High-Fidelity PCR Master Mix |
| Reaction Speed | Fast |
| Hot Start | No |
| Overhang | Blunt |
| Concentration | 2X |
| For Use With (Application) | Standard PCR,High-fidelity PCR |
Applied Biosystems™ TaqMan™ Fast Virus 1-Step Master Mix for qPCR
TaqMan Fast Virus 1-Step Master Mix is designed for fast, highly sensitive real-time RT-PCR, even in the presence of challenging PCR inhibitors.
| Shipping Condition | Dry Ice |
|---|---|
| Reverse Transcriptase | M-MLV |
| Form | Liquid |
| InhibitorToleranceSimple | High |
| For Use With (Application) | Microbial Detection,Gene Expression,DNA Quantitation,Presence/Absence |
| PCR Method | 1-step RT-qPCR |
| Multiplex Capability | Up to 3-plex |
| Detection Method | Primer-probe |
| Thermostability | Not Applicable |
| Polymerase | AmpliTaq Fast DNA Polymerase |
| Format | Tube |
| Product Type | 1-Step Master Mix |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudio 12k Flex, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio 7 Flex, StepOne, StepOnePlus, ViiA 7 System |
| Sample Type | Total RNA, Viral DNA, Viral RNA, mRNA |
| Reaction Speed | Fast or Standard |
| Target Specificity | RNA only, Both RNA and DNA |
| Hot Start | Built-In Hot Start |
| Product Line | TaqMan |
Invitrogen™ Quant-iT™ dsDNA Assay Kits, high sensitivity (HS) and broad range (BR)
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
For highly selective quantitation of double-stranded DNA over RNA, the Quant-iT dsDNA Assay Kits, high sensitivity and broad range, produce fluorescence signals that are linear in the ranges of 0.2-100 ng and 2-1000 ng of DNA, respectively.
| Shipping Condition | Room Temperature |
|---|---|
| No. of Reactions | 1000 Assays (200 μL assay volume) |
| For Use With (Equipment) | Microplate Reader |
| Quantity | 1 kit |
| Product Line | Quant-iT |
| Detection Method | Fluorescence |
Thermo Scientific™ GeneJET Genomic DNA Purification Kit
Thermo Scientific GeneJET Genomic DNA Purification Kit is designed for rapid and efficient purification of high quality genomic DNA from various mammalian cell culture and tissue samples, whole blood, bacteria and yeast.
| Column Type | Spin Column |
|---|---|
| High-throughput Compatibility | Not High-throughput Compatible (Manual) |
Invitrogen™ RNase Cocktail™ Enzyme Mix
Endonuclease-free, exonuclease-free, protease-free
| Shipping Condition | Dry Ice |
|---|---|
| Product Type | RNAse Cocktail Enzyme Mix |
| Enzyme | RNase |
| Product Line | Ambion |
Thermo Scientific™ RNase I (10 U/μL)
Ribonuclease I (RNase I) is an endoribonuclease that hydrolyzes single-stranded RNA to nucleoside 3'-monophosphates.
| Product Type | RNase I |
|---|---|
| Enzyme | RNase |
| Concentration | 10 U/μL |
Invitrogen™ AccuPrime™ Taq DNA Polymerase, High Fidelity
Invitrogen AccuPrime Taq DNA Polymerase, High Fidelity, provides qualified reagents for the high-fidelity PCR amplification of nucleic acid templates.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | AccuPrime Taq DNA Polymerase High Fidelity |
| GC-Rich PCR Performance | Low |
| Fidelity (vs. Taq) | 9X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | Separate Components |
| Product Type | DNA Polymerase |
| Reaction Speed | Standard |
| Hot Start | Built-In Hot Start |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |
| Detection Method | Primer-probe |
Invitrogen™ Quant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents
Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent.
| Shipping Condition | Room Temperature |
|---|---|
| Excitation/Emission | 500/525 |
| No. of Reactions | 200 Assays (2 mL assay volume) |
| Quantitation Range | 50 pg to 2 μg |
| For Use With (Equipment) | Microplate Reader |
| Product Line | PICOGREEN, Quant-iT |
| Detection Method | Fluorescence |
Thermo Scientific™ Phusion™ Plus PCR Master Mixes
Thermo Scientific Phusion Plus PCR Master Mix is a convenient, ready-to-use 2X mixture of Phusion Plus DNA Polymerase, buffer, magnesium chloride, and dNTPs.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Phusion Plus DNA Polymerase |
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | >100X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | Ready to use master mix |
| Reaction Speed | 15 to 30 sec./kb |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| Concentration | 2X |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Invitrogen™ Lipofectamine™ CRISPRMAX™ Cas9 Transfection Reagent
Lipofectamine CRISPRMAX Cas9 Transfection Reagent is an optimized lipid-based transfection reagent for CRISPR-Cas9 protein delivery, providing the cleavage efficiency of electroporation with simplicity and scalability.
| Format | 6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks |
|---|---|
| Product Type | Transfection Reagent |
| Transfection Technique | Lipid-based Transfection |
| Sample Type | Protein,Ribonucleoprotein,Cas9 Ribonucleoprotein,Cas9,Plasmid DNA |
| Classification | Animal Origin-Free |
| Serum Compatible | Yes |
| For Use With (Application) | Transfection |
| Product Line | CRISPRMAX, Lipofectamine |
| Cell Type | Established Cell Lines,Stem Cells,Primary Cells,Hard-to-Transfect Cells |
Thermo Scientific™ pBR322 Plasmid DNA
Optimize cloning with Thermo Scientific™ pBR322 DNA, a 4361 bp double-stranded closed circular medium copy plasmid DNA.
Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Buffer II and MgCl2
AmpliTaq Gold DNA Polymerase is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation.
| Polymerase | AmpliTaq Gold DNA Polymerase |
|---|---|
| Fidelity (vs. Taq) | 1X |
| Size (Final Product) | 5 kb or less |
| Product Type | DNA Polymerase |
| Overhang | 3'-A |
| Concentration | 5 U/μL |
| Exonuclease Activity | 5' - 3' |