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Real Time PCR Products

A range of products specifically designed for real-time PCR workflows, a process that monitors the amplification of a targeted DNA molecule during the PCR reaction, not at its end, as in conventional PCR. Products include assays, reagents, kits, and master mixes.
Real Time PCR Master Mixes (32)
Real Time PCR Reagents and Kits (12)

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1
Promotions Available

Applied Biosystems™ TaqMan™ Environmental Master Mix 2.0

Accurate, real-time PCR-based pathogen detection in the presence of high levels of inhibitors.

EDGE
Purity or Quality Grade UP (Ultra Pure)
Concentration 2X
For Use With (Application) Gene Expression,Genotyping,Pathogen Detection,Virus Detection
PCR Method qPCR
Detection Method Primer-probe
Polymerase AmpliTaq Gold DNA Polymerase
Format Bottle
Product Type qPCR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7000 System, 7300 System, 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, StepOne™, Standard Mode
Sample Type Environmental Sample, Food
Reaction Speed Standard
Hot Start Built-In Hot Start
Product Line TaqMan
2
Promotions Available

Applied Biosystems™ TaqPath™ qPCR Master Mix, CG
GREENER_CHOICE

TaqPath qPCR Master Mix, CG, is a 2X formulation designed for gene expression and miRNA analysis. It contains thermostable Fast DNA polymerase, uracil-N-glycosylase (UNG), dNTPs with dUTP, ROX dye (passive reference), and optimized buffer components for maximum robustness and reproducibility.

EDGE
Content And Storage Store at 2°C to 8°C in the dark.
Form Liquid
InhibitorToleranceSimple High
Concentration 2X
For Use With (Application) Gene Expression,Microbial Detection,DNA Quantification,Presence/Absence,miRNA analysis
PCR Method qPCR
Multiplex Capability Duplex
Detection Method Primer-probe
Includes dUTP, UNG/UDG
Thermostability 48 hr., Assembled Reaction
Polymerase Fast DNA Polymerase
Sensitivity Down to 10 copies
Format Bottle
Product Type qPCR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) QuantStudio Dx, 7500 Fast Dx
Sample Type cDNA, DNA
Reaction Speed Fast or Standard
Target Specificity cDNA or gDNA only
Hot Start Built-In Hot Start
Product Line TaqPath
3
Promotions Available

Applied Biosystems™ TaqMan™ Multiplex Master Mix

Applied Biosystems™ TaqMan™ Multiplex Master Mix is formulated to amplify up to 4 targets in a single reaction, delivering shorter run times (less than 40 minutes).

EDGE
Polymerase AmpliTaq Fast DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) 2 X
Product Type Multiplex Master Mix
Sample Type DNA
Reaction Speed Fast
Concentration 2X
For Use With (Application) Gene Expression,Virus Detection
PCR Method Multiplex qPCR
Product Line TaqMan
Detection Method Primer-probe
4
Promotions Available

Applied Biosystems™ TaqMan™ PreAmp Master Mix (cDNA pre-amplification for qPCR)

TaqMan™ PreAmp Master Mix pre-amplifies small amounts of cDNA without introducing amplification bias to the sample.

GC-Rich PCR Performance High
Product Type PreAmp Master Mix
For Use With (Equipment) 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System
Sample Type cDNA
For Use With (Application) Pre-amplification
PCR Method qPCR
Product Line TaqMan
Detection Method Primer-probe
5

Applied Biosystems™ resDNASEQ™ Quantitative Vero DNA Kits

The resDNASEQ Quantitative Vero DNA Kit is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from Vero (African Green Monkey) cells, an expression system commonly used for the production of vaccines.

No. of Reactions 100 Reactions
Format Kit
Label or Dye FAM
Quantity 100 reactions
Host Cell Vero
PCR Method qPCR
Product Line resDNASEQ
Detection Method Primer-probe
6

Applied Biosystems™ resDNASEQ™ Quantitative Plasmid DNA - Kanamycin Resistance Gene Kits

The resDNASEQ Quantitative Plasmid DNA - Kanamycin Resistance Gene Kit is a quantitative PCR (qPCR)-based system for the detection of residual kanamycin-resistant plasmids.

No. of Reactions 100 Reactions
Target Kanamycin Resistant Plasmids
Format Kit
Label or Dye FAM
Quantity 100 reactions
PCR Method qPCR
Product Line resDNASEQ
Detection Method Primer-probe
7

Applied Biosystems™ resDNASEQ™ Quantitative E. coli DNA Kits

This quantitative PCR (qPCR)-based kit, part of the resDNASEQ™ E. coli Residual DNA Quantitation System, enables highly sensitive quantitation of host cell DNA from E. coli expression systems, typically in less than 4 hours.

No. of Reactions 100 Reactions
Format Kit
Label or Dye FAM
Quantity 100 reactions
Host Cell E.coli
PCR Method qPCR
Product Line resDNASEQ
Detection Method Primer-probe
8

Applied Biosystems™ resDNASEQ™ Quantitative MDCK DNA Kits

The resDNASEQ™ Quantitative MDCK DNA Kit is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from MDCK (canine kidney) cells, an expression system commonly used for the production of vaccines.

No. of Reactions 100 Reactions
Format Kit
Label or Dye FAM
Quantity 100 reactions
Host Cell MDCK
PCR Method qPCR
Product Line resDNASEQ
Detection Method Primer-probe
9

Applied Biosystems™ resDNASEQ™ Human Residual DNA Quantitation Kits

The resDNASEQ Human Residual DNA Quantitation Kit is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from human cell lines used as expression systems for recombinant proteins.

No. of Reactions 100 Reactions
Format Kit
Label or Dye FAM
Quantity 100 reactions
Host Cell Human DNA
PCR Method qPCR
Product Line resDNASEQ
Detection Method Primer-probe
10

Applied Biosystems™ resDNASEQ™ Quantitative NS0 DNA Kits

The resDNASEQ™ Quantitative NS0 DNA Kit is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from NS0 (murine myeloma) cells, an expression system commonly used for the production of vaccines.

No. of Reactions 100 Reactions
Format Kit
Label or Dye FAM
Quantity 100 reactions
Host Cell NS0
PCR Method qPCR
Product Line resDNASEQ
Detection Method Primer-probe
11

Applied Biosystems™ resDNASEQ™ Quantitative Pichia pastoris DNA Kits

The resDNASEQ™ Quantitative Pichia pastoris DNA Kit is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from Pichia pastoris cells.

No. of Reactions 100 Reactions
Format Kit
Label or Dye FAM
Quantity 100 reactions
Host Cell Pichia
PCR Method qPCR
Product Line resDNASEQ
Detection Method Primer-probe
12

Applied Biosystems™ resDNASEQ™ Quantitative HEK293 DNA Kits

The resDNASEQ Quantitative HEK293 DNA Kit is a quantitative PCR (qPCR)-based system for the detection of host-cell DNA from HEK293 cell lines.

No. of Reactions 100 Reactions
Format Kit
Label or Dye FAM
Quantity 100 reactions
Host Cell HEK
PCR Method qPCR
Product Line resDNASEQ
Detection Method Primer-probe
13

Applied Biosystems™ ViralSEQ™ Quantitative Sf-Rhabdovirus Kits

The Applied Biosystems ViralSEQ Quantitative Sf-Rhabdovirus Kit is a two-step RT-qPCR assay for the detection of Sf-rhabdovirus in recombinant products manufactured in insect cell lines using baculovirus expression systems.

Shipping Condition Dry Ice
Target Sf-rhabdovirus
Quantity 100 reactions
PCR Method qPCR
Product Line ViralSEQ
Detection Method Primer-probe
14

Applied Biosystems™ resDNASEQ™ Quantitative Sf9 and Baculovirus DNA Kits

The resDNASEQ Quantitative Sf9 and Baculovirus DNA Kit is a duplex quantitative PCR (qPCR)-based system for the detection of residual DNA from the Sf9 baculovirus platform.

No. of Reactions 100 Reactions
Format Kit
Label or Dye FAM + VIC
Quantity 100 reactions
Host Cell SF9 + Bacoluvirus
PCR Method qPCR
Product Line resDNASEQ
Detection Method Primer-probe
15

Applied Biosystems™ resDNASEQ™ Quantitative E1A DNA Fragment Length Kits

The resDNASEQ Quantitative E1A DNA Fragment Length Kit is a quantitative PCR (qPCR)-based system that measures and quantitates the fragment lengths of residual host-cell DNA by targeting the E1A gene in HEK 293 cell lines.

No. of Reactions 100 Reactions
Format Kit
Label or Dye FAM
Quantity 100 reactions
Host Cell HEK
PCR Method qPCR
Product Line resDNASEQ
Detection Method Primer-probe