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Applied Biosystems™ TaqMan™ PreAmp Master Mix (cDNA pre-amplification for qPCR)
Shop All Applied Biosystems ProductsDescription
TaqMan™ PreAmp Master Mix lets you: • Analyze mRNA from precious samples such as laser capture microdissections, needle biopsies, and formalin-fixed paraffin-embedded tissues (FFPE) • Stretch as little as 1 ng of cDNA into 200 real-time PCR reactions for gene expression analysis using TaqMan™ Gene Expression assays • Analyze up to 100 gene expression targets with minimal hands-on time Preserves Equilibrium of Targets Without Preamplification Bias: In the past, preamplification kits and techniques often resulted in uneven amplification of targets and incorrect or biased data (as shown by low correlation coefficients between preamplified and unamplified samples). TaqMan™ PreAmp Master Mix amplifies with no bias and provides extremely high correlation between amplified and unamplified cDNA. Easy Workflow: With TaqMan™ PreAmp Master Mix, preamplification setup takes only a few minutes. After a one-time pooling of the TaqMan™ assays , preamplification setup (mixing the pooled assays with cDNA sample and TaqMan™ PreAmp Master Mix) takes only five additional minutes. Cycling takes 1.5 hours using a GeneAmp™ 9700 thermal cycler. For Research Use Only. Not for use in diagnostic procedures.
Order Info
Guaranteed minimum shelf life is 60 days.
Specifications
Specifications
| Content And Storage | Contains one 5-mL bottle of 2X master mix. Store at 2–8°C. Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA). |
| Detection Method | Primer-probe |
| Format | Bottle |
| GC-Rich PCR Performance | High |
| PCR Method | qPCR |
| Reaction Speed | Standard |
| For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System |
| Product Line | TaqMan |
| Product Type | PreAmp Master Mix |
| Quantity | 200 reactions |
| Show More |
For Research Use Only. Not for use in diagnostic procedures.
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