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Chromatography Media

Resins or particles used to separate components of a sample via column chromatography; products include media used in cation or anion exchange, fast flow, and high-performance chromatography techniques, etc.
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1

Cytiva Sephaorose™ Fast Flow Anion Exchange Media: DEAE

Used for preparative protein separations in both research and industrial applications. Cytiva Life Sciences™ Sephaorose™ Fast Flow Anion Exchange Media: DEAE ensures low levels of non-specific binding.

2

Cytiva Butyl-S Sepharose™ 6 Fast Flow Chromatography Media

The least hydrophobic medium in the Sepharose Fast Flow hydrophobic interaction chromatography (HIC) range and is particularly useful during the initial stages of purification process

3

Cytiva Strep-Tactin™ XT Sepharose™ Chromatography Resin

Chromatography resin for purification of recombinant proteins tagged with a Strep-tag™ II and Twin-Strep-tag™.

EDGE
Particle Size 34 μm
Ligand Type Strep-Tactin XT
Density (Metric) Approx. 5 mg/mL resin
Matrix Rigid, cross-linked agarose, spherical
Stability Stable in all commonly used aqueous buffers
pH Range 6 to 10 pH
Binding Capacity Approx. 10 mg protein with Strep-tag II or Twin-Strep-tag/mL of resin
Storage Requirements 2 to 8°C in 20% ethanol
For Use With (Application) Purification of recombinant proteins tagged with Strep-tag II and Twin-Strep-tag
4

Cytiva HiTrap™ TALON™ Crude Prepacked Columns

For purification of histidine-tagged recombinant proteins by immobilized metal affinity chromatography (IMAC). Cytiva Lifescience™ HiTrap™ TALON™ Crude Prepacked Columns offer a cobalt-based medium allowing protein purification under native or denaturing conditions.

EDGE
Particle Size 60 to 160 μm
Ligand Type Talon Superflow Cobalt-IMAC
Flow Rate 1 to 4 mL/min
Matrix 6% Cross-Linked Agarose
Storage Buffer 20% Ethanol
pH Range 3 to 12
Product Type HiTrap Talon crude prepacked Column
Material Polypropylene
Storage Requirements 4°C to 8°C
For Use With (Application) For PurifiCatIon of Histidine-Tagged Recombinant Proteins by Immobilized Metal Affinity Chromatography, Used Buffers and Easily Regenerated with 0.5 M NaOH
Max. Pressure 3 bar
5

Applied Biosystems™ Trifluoroacetic Acid (TFA) Neat Liquid

Trifluoroacetic Acid (TFA, concentrated) for use with OPC™ cartridges for oligonucleotide purification.

6

Cytiva His SpinTrap™ TALON™

Single-use spin column for high purity purification of histidine-tagged proteins

7

Cytiva rProtein A Sepharose™ Fast Flow Affinity Media

Designed for purification of monoclonal and polyclonal antibodies at labs and process scales

Particle Size 60 to 165 μm
Ligand Type Recombinant Protein A (E. coli)
Flow Rate 150 to 250 cm/Hoμr
Matrix 4% Cross-Linked Agarose
Stability Stable in aqueous Buffers commonly used in Protein A Chromatography
Storage Buffer 20% Ethanol
pH Range 2 to 9
Product Type rProtein A Sepharose Fast Flow Media
Height (English) Bed 9.84 in.
Storage Requirements 2°C to 8°C
For Use With (Application) For PurifiCatIon of Monoclonal and Polyclonal Antibodies at Labs and Process Scales
Height (Metric) Bed 25 cm
Max. Pressure 100 kPa
8

Cytiva NHS-Activated Sepharose™ 4 Fast Flow Affinity Media

Use for coupling amino-containing smaller proteins and peptides. Cytiva Life Sciences™ NHS-Activated Sepharose™ 4 Fast Flow Affinity Media yields coupled products that are very stable, especially at high pH.

9

Cytiva Sepharose™ Fast Flow Cation Exchange Media

SP Sepharose Fast Flow is a sulphopropyl (SP) strong cation exchange chromatography resin for fast protein purification. CM Sepharose Fast Flow is a weak cation exchanger based on the well established Sepharose Fast Flow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.

Particle Size 45 to 165 μm
pH Range 4 to 13
10

Cytiva TALON™ Superflow Medium

Offers different selectivity compared to nickel-charged media. Cytiva TALON™ Superflow Medium used for purification of histidine-tagged recombinant proteins by IMAC.

Particle Size 60 to 160 μm
Ligand Type Talon Superflow Cobalt-IMAC
Matrix 6% Cross-Linked Agarose
Storage Buffer 20% Ethanol
pH Range 3 to 12
Product Type Talon Superflow Media
Binding Capacity Up to 20 mg Histidine-tagged Protein
Storage Requirements 4°C to 8°C
For Use With (Application) For PurifiCatIon of Histidine-Tagged Recombinant Pby Immobilized Metal Affinity Chromatography
13

Cytiva Phenyl Sepharose™ 6 Fast Flow Chromatography Media

Used for capture and intermediate purification requiring low to medium hydrophobicity

Ligand Type Phenyl
Matrix 6% Cross-Linked Agarose
pH Range 3 to 13
For Use With (Application) For Capture and Intermediate PurifiCatIon Requiring Low to Medium Hydrophobicity
14

Cytiva Capto™ Multimodal (MMC) Chromatography Media

Used to solve specific purification challenges at both high and low ionic strengths

Particle Size 75 μm
Product Type Capto MMC Media
15

Cytiva Heparin Sepharose™ 6 Fast Flow Affinity Chromatography Media

USe for separations of biomolecules with an affinity for heparin, including antithrombin III, coagulation factors and other plasma proteins. Cytiva Life Sciences™ Heparin Sepharose™ 6 Fast Flow Affinity Chromatography Media is fast and reliable and highly suitable for process-scale purification processes.