Restriction Enzymes

Restriction enzymes cleave DNA into fragments at or near specific recognition sequences known as restriction sites; also known as restriction endonuclease, or restrictase. Products include various restriction enzymes, kits, buffers, etc.

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Thermo Scientific™ TheraPure™ Restriction Enzymes

Thermo Scientific TheraPure in vitro transcription (IVT) enzymes are designed for advanced research and early development of mRNA therapeutics and vaccines.

Pricing and Availability
Specifications

Cut Site	CTCTTC(1/4)^
Content And Storage	Store at -20°C.
Form	Liquid
Enzyme	Eam1104I
Methylation Sensitivity	Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Quantity	3000 U, 150 μL
Type IIS RE	Yes
Incubator Temperature	37°C
Concentration	20 U/μL
For Use With (Application)	In vitro Transcription
Inactivation Temperature	65°C
Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Research Category	Drug Development
Compatible Buffer	10X Tango Buffer
Optimal Reaction Temperature	37°C
Product Line	TheraPure

Thermo Scientific™ Dilution Buffer for Restriction Enzymes

Optimize applications that require diluted enzymes with this dilution buffer.

Pricing and Availability

Thermo Scientific™ Buffer Set for Restriction Enzymes

Ensure the optimum reaction conditions for each restriction enzyme with this five buffer system.

Pricing and Availability

Thermo Scientific™ EcoRI (10 U/μL)

The EcoRI restriction enzyme recognizes G^AATTC sites and cuts best at 37°C in its own unique buffer.

Pricing and Availability
Specifications

Concentration	10 U/μL

Thermo Scientific™ DpnI (10 U/μL)

Cut at Gm6A^TC sites with DpnI restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: MalI).

Pricing and Availability
Specifications

Concentration	10 U/μL

Thermo Scientific™ FastDigest BamHI

Cut at G^GATCC sites with Thermo Scientific™ FastDigest™ BamHI restriction enzyme, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.

Pricing and Availability
Specifications

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	BamH I
Methylation Sensitivity	Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	10X FastDigest Buffer/FastDigest Green Buffer
Overhang	5' protruding ends
Type IIS RE	No
Optimal Reaction Temperature	37°C
Inactivation Temperature	80°C
Product Line	FastDigest

Thermo Scientific™ BglII (10 U/μL)

Cut at A^GATCT sites with BglII restriction enzyme, which performs best at 37°C in O buffer.

Pricing and Availability
Specifications

Concentration	10 U/μL

Thermo Scientific™ PvuII (10 U/μL)

The PvuII restriction enzyme recognizes CAG^CTG sites and cuts best at 37°C in G buffer.

Pricing and Availability
Specifications

Concentration	10 U/μL

Thermo Scientific™ DraI (10 U/μL)

Thermo Scientific™ DraI is available as a FastDigest™ enzyme which is used for rapid DNA digestion.

Pricing and Availability

Thermo Scientific™ FastDigest™ NheI: PROMO

Thermo Scientific FastDigest NheI restriction enzyme recognizes G^CTAGC site and cuts best at 37°C in 5 to 15 minutes using universal FastDigest Buffer. Isoschizomers: AsuNHI, BmtI.

Pricing and Availability

Thermo Scientific™ HinfI (10 U/μL)

The HinfI restriction enzyme recognizes G^ANTC sites and cuts best at 37°C in R buffer.

Pricing and Availability
Specifications

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	Hinf I
Methylation Sensitivity	CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	10X Buffer R
Type IIS RE	No
Optimal Reaction Temperature	37°C
Concentration	10 U/μL

Thermo Scientific™ FastDigest XhoI

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.

Pricing and Availability
Specifications

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	Xho I
Methylation Sensitivity	CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	10X FastDigest Buffer/FastDigest Green Buffer
Overhang	5' protruding ends
Type IIS RE	No
Optimal Reaction Temperature	37°C
Inactivation Temperature	80°C
Product Line	FastDigest

Thermo Scientific™ TatI (5 U/μL)

Cut at W^GTACW sites with TatI restriction enzyme, which performs best at 65°C in Tango buffer.

Pricing and Availability

Thermo Scientific™ SgsI (AscI) (10 U/μL)

The SgsI (AscI) restriction enzyme recognizes GG^CGCGCC sites and cuts best at 37°C in Tango buffer (Isoschizomers: AscI, PalAI).

Pricing and Availability
Specifications

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	SgsI (AscI)
Methylation Sensitivity	CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	10X Buffer Tango
Type IIS RE	No
Optimal Reaction Temperature	37°C
Concentration	10 U/μL

Thermo Scientific™ MssI (PmeI) (5 U/μL)

The MssI (PmeI) restriction enzyme recognizes GTTT^AAAC sites and cuts best at 37°C in B buffer (Isoschizomers: PmeI).

Pricing and Availability
Specifications

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	MssI (PmeI)
Methylation Sensitivity	Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	10X Buffer B
Type IIS RE	No
Optimal Reaction Temperature	37°C
Concentration	5 U/μL

Restriction Enzymes

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