Cell Based Assays
Invitrogen™ Calcein AM, Cell-permeant Green and Blue Dyes
Calcein AM is a cell-permeant dye that can be used to determine cell viability in most eukaryotic cells. In live cells, the non-fluorescent Calcein AM is converted to green or blue fluorescent Calcein, after acetoxymethyl ester hydrolysis by intracellular esterases.
Invitrogen™ SYTO™ Green Fluorescent Nucleic Acid Stains
SYTO Green Fluorescent Nucleic Acid Stains exhibit bright, green fluorescence upon binding to nucleic acids. These dyes are cell-permeant and can be used to stain RNA and DNA in live cells, dead cells, and bacteria.
Molecular Probes™ LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit, for 405 nm excitation
The LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit is used to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining, or prior to elimination of biohazardous materials using formaldehyde fixation.
Invitrogen™ CellMask™ Plasma Membrane Stains
Invitrogen CellMask™ plasma membrane stains enable fast and uniform labeling of the plasma membrane in almost all cell types. These stains work rapidly and can be detected using standard microscope filter sets with any imaging instrument.
Invitrogen™ LIVE/DEAD™ Cell Imaging Kit (488/570)
Sensitive two-color fluorescence cell viability assay optimized for FITC and Texas Red™ filters
Invitrogen™ CyQUANT™ LDH Cytotoxicity Assays
CyQUANT cytotoxicity assays measure cellular toxicity based on the release of cytosolic enzymes (LDH or G6PD) from damaged cells. These cytotoxicity assays utilize either colorimetric or fluorescence detection and allow continuous monitoring of cell death using microplate readers.
Invitrogen™ Oxidized Low-density Lipoprotein (OxLDL) and DiI-OxLDL
Study lipid transport and atherosclerosis with oxidized LDL (OxLDL) and the fluorescently labeled DiI-OxLDL. Oxidized forms of human low-density lipoprotein (LDL) are used to examine scavenger receptor-mediated endocytosis of OxLDL by macrophages and endothelial cells.
Invitrogen™ LysoTracker™ Deep Red
Deep red-fluorescent dye for labeling and tracking acidic organelles in live cells
Molecular Probes™ Fluo-4, AM, cell permeant
Fluo-4 is an analog of fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels.
Invitrogen™ CyQUANT™ Cell Proliferation Assays
CyQUANT cell proliferation assays are fast and highly sensitive fluorescence methods for counting cells to assess cell growth and cytotoxicity in microplate format. These assays use fluorescent nucleic-acid-binding dyes to measure cell number based on DNA content.
Invitrogen™ Amplex™ Red and Amplex™ UltraRed Reagents
The Amplex Red and UltraRed reagents are fluorogenic substrates for peroxidase. These highly sensitive and stable probes react with H2O2 in the presence of HRP for solution-based assays and ELISAs. Amplex UltraRed is a brighter version of Amplex Red with an improved pH range and sensitivity.
Invitrogen™ Click-iT™ EdU Cell Proliferation Kit for Imaging, Alexa Fluor™ 647 dye
Works the first time, every time, in less time
Invitrogen™ ReadyProbes™ F-Actin Labeling Reagents
ActinRed™ and ActinGreen™ ReadyProbes™ reagents assist with bright, selective F-actin labeling in fixed cells and tissues. The ready-to-use dropper or dual-use bottle enables dropwise dispensing or direct pipetting for fast, flexible cytoskeleton imaging.
Invitrogen™ Image-iT™ TMRM Reagent (mitochondrial membrane potential indicator)
Healthy mitochondrial membranes maintain a difference in electrical potential between the interior and exterior of the organelle, referred to as a membrane potential.
Invitrogen™ PrestoBlue™ and PrestoBlue™ HS Cell Viability Reagents
Monitor cell proliferation and viability with the resazurin-based PrestoBlue and PrestoBlue HS cell viability reagents. These nontoxic and cell permeable reagents quantitatively measure cell viability in as little as 10 minutes using absorbance- or fluorescence-based microplate readers.