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Real Time PCR Assays and Arrays

Array cards and plates, endogenous controls, kits, and other PCR products designed to be used while performing quantitative real-time PCR assays. Ideal for various applications including gene-expression research and diagnostic testing.
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Promotions Available

Applied Biosystems™ TaqMan™ Gene Expression Assays

Applied Biosystems TaqMan Gene Expression assays are used for quantitative real-time PCR or digital PCR analysis of gene expression and consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (FAM) on the 5' end and a minor groove binder (MGB) and non-fluorescent quencher (NFQ) on the 3' end.

Shipping Condition Room Temperature
GC-Rich PCR Performance High
Fidelity (vs. Taq) 60 X
Product Type Gene Expression Assay
Reaction Speed Fast
PCR Method qPCR
Product Line TaqMan
Detection Method Primer-probe
2
Promotions Available

Applied Biosystems™ TaqMan™ MGB Probe

Custom TaqMan probes are dual-labeled primers suitable for use in any real-time PCR application. They incorporate a 5' reporter dye and a 3' nonfluorescent quencher/minor groove binder (MGB). Choose among FAM, VIC, ABY, JUN, Cy5, TET, and NED as reporters.

Purification Method HPLC
Purity or Quality Grade HPLC
Format Tube
Internal Probe Modification None
For Use With (Equipment) 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex, QuantStudio™ Absolute Q Digital PCR System
Form Liquid
3'Primer Modification MGB-NFQ
For Use With (Application) Real Time PCR (qPCR)
Product Line TaqMan
3
Promotions Available

Applied Biosystems™ Human GAPD (GAPDH) Endogenous Control (VIC™/MGB probe, primer limited)

Human GAPD (GAPDH) Endogenous Control (VIC™/MGB probe, primer limited)

4
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Applied Biosystems™ Human ACTB (Beta Actin) Endogenous Control (VIC™/MGB probe, primer limited)

Human ACTB (Beta Actin) Endogenous Control (VIC™/MGB probe, primer limited)

5

Applied Biosystems™ Human PPIA (Cyclophilin A) Endogenous Control (VIC™/MGB probe, primer limited)

Human PPIA (Cyclophilin A) Endogenous Control (VIC™/MGB probe, primer limited)

EDGE
6
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Applied Biosystems™ Mouse GAPD (GAPDH) Endogenous Control (VIC™/MGB probe, primer limited)

Mouse GAPD (GAPDH) Endogenous Control (VIC™/MGB probe, primer limited)

EDGE
7
Promotions Available

Applied Biosystems™ Protein Thermal Shift™ Starter Kit

This solution significantly reduces the time and cost associated with screening samples relative to currently available methods.

EDGE
8

Invitrogen™ Uracil DNA Glycosylase

Removes uracil residues from sugar moiety of single- and double-stranded DNA without destroying phosphodiester backbone

EDGE
9
Promotions Available

Applied Biosystems™ Eukaryotic 18S rRNA Endogenous Control (FAM™/MGB probe, non-primer limited)

Eukaryotic 18S rRNA Endogenous Control (FAM™/MGB probe, non-primer limited)

EDGE
Shipping Condition Room Temperature
Content And Storage Control Primer-Probe Set(s)
Gene Symbol 18S
Amplicon Size 187
Concentration 20X
For Use With (Application) Gene Expression
PCR Method 2-step RT-qPCR
Detection Method Primer-probe
Accession Number X03205.1
Target 18S rRNA
Format Tube
Label or Dye FAM
Species Eukaryotic, Rat, Mouse, Human
Product Line FAM, TaqMan
10
Promotions Available

Applied Biosystems™ TaqMan™ RNase P Detection Reagents Kit

TaqMan™ RNase P Detection Reagents Kit

11
Promotions Available

Applied Biosystems™ TaqMan™ MicroRNA Assay

Applied Biosystems TaqMan MicroRNA Assays quantitate miRNAs with the specificity and sensitivity of TaqMan Assay chemistry. A simple, two-step protocol requires only reverse transcription with an miRNA-specific primer, followed by real-time PCR with TaqMan probes.

Shipping Condition Room Temperature
GC-Rich PCR Performance High
Content And Storage • 1 tube containing RT primer at concentration of 5X (XS and S sizes), 20X (M size), or 60X (L size)
• 1 tube containing a 20X (XS, S, and M sizes) or 60X (L size) mix of pre-formulated assay (1 probe and 2 primers).

Store at -15 to -25°C.
Format Tube
For Use With (Equipment) 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex, QuantStudio™ Absolute Q Digital PCR System
Reaction Speed Fast
PCR Method qPCR
Product Line TaqMan
12
Promotions Available

Applied Biosystems™ TaqMan™ SNP Genotyping Assays

5' nuclease assay to discriminate between two alleles of a specific SNP for use in genotyping studies.

For Use With (Application) Genotyping
13
Promotions Available

Applied Biosystems™ TaqMan™ Copy Number Reference Assay, human, RNase P

Human TaqMan™ Copy Number Reference Assays are run with human TaqMan™ Copy Number Assays in a duplex real-time PCR reaction to detect and measure copy number variations (CNVs) and smaller regions in the human genome.

Shipping Condition Ambient Temperature
GC-Rich PCR Performance Low
Content And Storage Control Primer-Probe Set(s)
Purity or Quality Grade RNase-Free, DNase-Free
Internal Probe Modification TAMRA™ Quencher (3'), VIC™ (5')
Form Frozen
Gene Symbol RPPH1
Concentration 20X
PCR Method qPCR
Detection Method Primer-probe
Target RNase P
Purification Method HPLC
Format Tube
Product Type Configurable Assay
Reaction Speed Standard
5'Primer Modification None
Label or Dye VIC
3'Primer Modification None
Species Human
Product Line TaqMan
14
Promotions Available

Applied Biosystems™ Protein Thermal Shift™ Dye Kit

This solution significantly reduces the time and cost associated with screening samples relative to currently available methods.

15
Promotions Available

Applied Biosystems™ Custom TaqMan™ Gene Expression Assays

Applied Biosystems Custom TaqMan Gene Expression assays are the custom version of our predesigned TaqMan assays for quantitative real-time PCR.

Shipping Condition Room Temperature
Content And Storage 1 tube containing a 20X (S and M sizes) or 60X (L size) mix of pre-formulated assay (1 probe and 2 primers).

Store at -15 to -25°C.
Product Type Custom TaqMan Gene Expression
For Use With (Equipment) 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex
Product Line TaqMan