Gel Electrophoresis Equipment and Supplies
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Invitrogen™ NuPAGE™ Bis-Tris Mini Protein Gels, 4–12%, 1.0–1.5 mm
Invitrogen NuPAGE Bis-Tris protein gels are precast polyacrylamide gels that provide broad molecular weight protein separation with high resolution and sample integrity.
| Shipping Condition | Room Temperature |
|---|---|
| Gel Percentage | 4 to 12% |
| Mode of Separation | Molecular Weight |
| Separation Range | 3.5 to 260 kDa |
| Gel Type | Bis-Tris |
| Length (Metric) | 8 cm |
| Separation Type | Denaturing |
| Storage Requirements | Store at 4–25°C. Do not freeze. |
| Shelf Life | 12 Months |
| Width (Metric) | 8 cm |
| Gel Size | Mini |
Invitrogen™ NuPAGE™ Bis-Tris Midi Protein Gels, 4 to 12%, 1.0 mm
Invitrogen NuPAGE Bis-Tris protein gels are precast polyacrylamide gels that provide broad molecular weight protein separation with high resolution and sample integrity.
| Shipping Condition | Room Temperature |
|---|---|
| Separation Range | 3.5 to 260 kDa |
| Length (Metric) | 8 cm |
| Quantity | 10 gels (1 box) |
| Storage Requirements | Store at 4–25°C. Do not freeze. |
| Width (Metric) | 13 cm |
| Gel Size | Midi |
| Gel Percentage | 4 to 12% |
| Mode of Separation | Molecular Weight |
| Gel Type | Bis-Tris |
| Separation Type | Denaturing |
| Recommended Applications | Denaturing |
| GelThickness | 1.0 mm |
| Shelf Life | 12 Months |
| Product Line | NuPAGE |
Invitrogen™ 10X Bolt™ Sample Reducing Agent
Optimized for use with Bolt™ Bis-Tris Plus gels, and available in a variety of formats.
| Content And Storage | Sample Reducing Agent (10X) contains 500 mM dithiothreitol (DTT). Store at 2°C to 8°C. |
|---|---|
| For Use With (Equipment) | Bolt Mini Gel Tank |
| Product Type | Sample Reducing Agent |
| Form | Liquid |
| Formulation | 500 mM dithiothreitol (DTT) |
| Reagent Type | Reducing Agent |
| Concentration | 10X, 500 mM |
| Gel Compatibility | Bolt™ Bis-Tris Plus Gels |
| Product Line | Bolt |
Invitrogen™ HiMark™ Pre-stained Protein Standard
Designed for analysis of high molecular weight proteins on NuPAGE™ Novex™ Tris-Acetate gels
Invitrogen™ 100 bp DNA Ladder
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This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Invitrogen 100 bp DNA Ladder is designed for sizing and approximate quantification of double-stranded DNA in the range of 100 bp to 2,000 bp.
| Shipping Condition | Approved for shipment at Room Temperature or on Dry Ice |
|---|---|
| Gel Type | Agarose |
| Sample Loading Volume | 1 mL |
| Product Type | DNA Ladder |
| Technology | Individual chromatography-purified DNA fragments |
| Ready to Load | No |
| Concentration | 0.5 μg/μL |
| Size Range | 100 to 2000 bp |
| Gel Compatibility | Agarose gel |
Invitrogen™ E-Gel™ SizeSelect™ II Agarose Gels, 2%
Most convenient method for size selection of DNA libraries for Next Generation Sequencing applications. Rocover your DNA library in 3 simple steps.
Invitrogen™ Digitonin (5%)
Digitonin (5%), or digitin, is a non-ionic detergent with a cholesterol-like structure that can water-solubilize lipids, permeabilize cells, be used as a cytotoxicity positive control and solubilize membrane-bound proteins for NativePAGE™ protein analysis or cryo-EM structural analysis.
Invitrogen™ NuPAGE™ Bis-Tris Mini Protein Gels, 12%, 1.0 mm
NuPAGE™ Novex™ 12% Bis-Tris Gels are pre-cast polyacrylamide gels designed to give optimal separation of small- to medium-sized proteins under denaturing conditions.
| Shipping Condition | Room Temperature |
|---|---|
| Gel Percentage | 12% |
| Mode of Separation | Molecular Weight |
| Gel Type | Bis-Tris |
| Length (Metric) | 8 cm |
| Separation Type | Denaturing |
| GelThickness | 1.0 mm |
| Storage Requirements | Store at 4–25°C. Do not freeze. |
| Shelf Life | 12 Months |
| Width (Metric) | 8 cm |
| Gel Size | Mini |
| Product Line | NuPAGE |
Invitrogen™ Gel Loading Buffer II (Denaturing PAGE)
A 1–2X solution of 95% Formamide, 18 mM EDTA, and 0.025% SDS, Xylene Cyanol, and Bromophenol Blue.
| Shipping Condition | Room Temperature |
|---|---|
| Content And Storage | Store at –20°C. |
| Format | Bottle |
| Form | Liquid |
| Gel Compatibility | Denaturing Polyacrylamide Gels |
| Product Line | Ambion |
Invitrogen™ Bolt™ Transfer Buffer (20X)
Optimized for the transfer of proteins from Bolt Bis-Tris Plus gels to membranes for western blotting
| Buffer | Transfer Buffers |
|---|---|
| Content And Storage | Store at 4 to 25°C. |
| Product Type | Western Blot Transfer Buffer |
| Form | Liquid |
| Concentration | 20 X |
| Gel Compatibility | Bolt™ Bis-Tris Plus Gels |
| Product Line | Bolt |
Invitrogen™ Bolt™ Bis-Tris Plus Mini Protein Gels, 12%, 1.0 mm, WedgeWell™ format
Invitrogen Bolt Bis-Tris Plus gels are precast polyacrylamide gels designed for optimal separation of a wide range of protein sizes under denaturing conditions. Bolt gels are ideal for western blot transfer and analysis, and any other techniques where protein integrity is crucial.
| Shipping Condition | Room Temperature |
|---|---|
| Separation Range | 3.5 to 80 kDa |
| Length (Metric) | 8 cm |
| Quantity | 10 gels (1 box) |
| Storage Requirements | Store at 4–25°C. Do not freeze. |
| Width (Metric) | 8 cm |
| Gel Size | Mini |
| Gel Percentage | 12% |
| Mode of Separation | Molecular Weight |
| Gel Type | Bis-Tris Plus |
| Separation Type | Denaturing |
| Recommended Applications | Denaturing |
| GelThickness | 1.0 mm |
| Shelf Life | 16 Months |
| Product Line | Bolt, WedgeWell |
Invitrogen™ Bolt™ Bis-Tris Plus Mini Protein Gels, 10%, 1.0 mm, WedgeWell™ format
Similar to NuPAGE™ Bis-Tris gels, Bolt™ Bis-Tris Plus gels are designed to deliver consistent gel performance and provide a neutral pH environment that minimizes protein modifications.
| Shipping Condition | Room Temperature |
|---|---|
| Separation Range | 3.5 to 260 kDa |
| Length (Metric) | 8 cm |
| Quantity | 10 gels (1 box) |
| Storage Requirements | Store at 4–25°C. Do not freeze. |
| Width (Metric) | 8 cm |
| Gel Size | Mini |
| Gel Percentage | 10% |
| Mode of Separation | Molecular Weight |
| Gel Type | Bis-Tris Plus |
| Separation Type | Denaturing |
| Recommended Applications | Denaturing |
| GelThickness | 1.0 mm |
| Shelf Life | 16 Months |
| Product Line | Bolt, WedgeWell |
Invitrogen™ DDM (n-dodecyl β-D-maltoside) (10%)
Detergent solution used to prepare native protein samples for use with the NativePAGE™ Gel System
Thermo Scientific™ PageRuler™ Plus Prestained Protein Ladder, 10 to 250 kDa
Thermo Scientific™ PageRuler Plus Prestained Protein Ladder is a mixture of nine (9) blue-, orange- and green-stained proteins (10 to 250 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.
| Shipping Condition | Approved for shipment on Wet or Dry Ice |
|---|---|
| Number of Markers | 9 |
| Product Type | Protein Ladder |
| Molecular Weight (g/mol) | 250, 130, 100, 70, 55, 35, 25, 15, 10 kDa |
| Ready to Load | Yes |
| Stain Type | Blue, Orange, Green |
| Size Range | 10 to 250 kDa |
| Gel Compatibility | Bolt™ Bis-Tris Plus Gels, Novex™ Tricine Gels, Novex™ Tris-Glycine Gels, NuPAGE™ Bis-Tris Gels, NuPAGE™ Tris-Acetate Gels, SDS-PAGE Gels |
| Product Line | PageRuler |
| Detection Method | Colorimetric, NIR Fluorescence (700 nm), RGB Fluorescence (555 nm) |