Enzyme und Inhibitoren

Invitrogen™ CIAP (Calf Intestinal Alkaline Phosphatase)

Phosphomonoesterase that removes 3' and 5' phosphates from DNA and RNA Calf Intestinal Alkaline Phosphatase, 1000 units(20 units/ µl)

Thermo Scientific™ T4 DNA-Ligase

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. T4 DNA LIGASE 1U/UL 2X500Ustorage temp: -20°C, 5x200 units (1u/µl)

Thermo Scientific™ DNase I, RNase-frei

An endonuclease that nonspecifically cleaves single- and double-stranded DNA to release di-, tri-, and oligonucleotides with 5'-phosphate and 3'-OH groups. DNASE I RNASE-FRE 50U/UL 1000U

Thermo Scientific™ T4 Polynukleotid-Kinase (T4 PNK)

T4 Polynucleotide Kinase (T4 PNK) catalyzes the transfer of the gamma-phosphate from ATP to the 5'-OH group of oligonucleotides, ss and dsDNA and RNA. T4 PNK 500U 10U/UL

Thermo Scientific™ Terminale Desoxyribonukleotidyltransferase (TdT)

Terminal Deoxynucleotidyl Transferase (TdT) catalyzes the template-independent addition of deoxyribonucleotides to the 3'-OH terminus of DNA molecules. TDT 20U/UL 2500U

Invitrogen™ TURBO DNA-free Kit

Ideal tool for clearing residual DNA Ambion® TURBO DNA-free, 50 reactions

Invitrogen™ Clonase™ Gateway™ LR Clonase II Enzyme Mix

Enzymes and buffer in single mix Gateway LR Clonase II Enzyme Mix, 100 reactions

Invitrogen™ DNase I

Purified from bovine pancreas DNase I, 20000 units,(Deoxyribonuclease I)(non-RNA prep.to remove contaminating DNA)

Thermo Scientific™ RNase A, DNase- und proteasefrei

Specifically degrade single-stranded RNA at C and U residues with the endoribonuclease RNase A. RNASE A DNASE FRE 10MG/ML 10MG

Invitrogen™ Recombinant RNase A

Rigorously tested for contaminating nonspecific endonuclease, exonuclease, and protease activity Recombinant RNase A,200 µg

Invitrogen™ Clonase™ Gateway™ BP Clonase II Enzyme Mix

Catalyzes in vitro recombination of PCR products Gateway BP Clonase II Enzyme Mix, 100 reactions

Invitrogen™ UltraPure™ Glycogen

Used as carrier for nucleic acid precipitation in ethanol UltraPure Glycogen, 100 µl

Invitrogen™ DNA-free™ DNA Removal Kit

Safely eliminate DNA contamination from RNA samples DNA-free DNase Treatment and Removal Reagent, 50rxns

Thermo Scientific™ Esp3I (BsmBI)

The Esp3I (BsmBI) restriction enzyme recognizes CGTCTC(1/5)^ sites and cuts best at 37°C in Tango (+DTT) buffer (Isoschizomers: BsmBI). ESP3I (BSMBI) 10U/UL 1000U

Thermo Scientific™ NotI

The NotI restriction enzyme recognizes GC^GGCCGC sites and cuts best at 37°C in O buffer (Isoschizomers: CciNI). NOTI 50U/UL 1500U

Thermo Scientific™ FastDigest SphI (PaeI)

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer. FASTDIGEST SPHI (PAEI) 20UL

Thermo Scientific™ 10x Reaktionspuffer mit MgCl2 für DNase I

Optimize reaction condions for RNase-free DNase I with this 10X reaction Tris-HCl (pH 7.5) buffer containing MgCl2. 10X DNASE I RNASE-FREE 1ML

Thermo Scientific™ Exonuklease I (Exo I)

Degrade single-stranded DNA in a 3'→5' direction with Exonuclease I (ExoI), leaving 5'-terminal dinucleotides intact. EXO I 20U/UL 20000U

Invitrogen™ Terminal Deoxynucleotidyl Transferase, Recombinant

Performance and quality tested Terminal Deoxynucleotidyl Transferase,Recombinant, 500 units

Thermo Scientific™ FastDigest BbsI (BpiI)

Cut at GAAGAC(2/6)^ sites with Thermo Scientific™ FastDigest™ BbsI (BpiI), which performs best using universal FastDigest Buffer (Isoschizomers: BpuAI, BstV2I). FASTDIGEST BBSI (BPII) 20UL

Thermo Scientific™ Agarase

Specifically digests the agarose polysaccharide core, which allows for gentle yet efficient recovery of DNA or RNA fragments from low melting point agarose. AGARASE 0.5U/UL 100U

Thermo Scientific™ PaeI (SphI)

The PaeI (SphI) restriction enzyme recognizes GCATG^C sites and cuts best at 37°C in B buffer (Isoschizomers: BbuI, SphI). PAEI (SPHI) 10U/UL 2500U

Thermo Scientific™ RiboLock RNase-Inhibitor

RiboLock RNase Inhibitor specifically inhibits the activity of RNases A, B, and C, protecting RNA under a variety of reaction conditions. RIBOLOCK RI 40U/UL 24X2500U

Thermo Scientific™ FastDigest Bsu36I (Eco81I)

Cut at CC^TNAGG sites with Thermo Scientific™ FastDigest™ Bsu36I (Eco81I), which performs best using universal FastDigest Buffer (Isoschizomers: AxyI, Bse21I). FASTDIGEST BSU36I(ECO81I) 50UL

Thermo Scientific™ FastDigest BspHI (PagI)

Cut at T^CATGA sites with Thermo Scientific™ FastDigest™ BspHI (PagI), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: CciI, RcaI). FASTDIGEST BSPHI (PAGI) 50UL

Invitrogen™ Anza™ 10 DpnI

Simplify traditional restriction enzyme cloning with the Invitrogen™ Anza™ Restriction Enzyme Cloning System. Anza 10 DpnI (4000 units)

Thermo Scientific™ FastDigest™ DpnI

Cut at Gm6A^TC sites with Thermo Scientific™ FastDigest™ DpnI, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: MalI). FASTDIGEST DPNI 100UL

Thermo Scientific™ Bsp1407I (BsrGI)

Cut at T^GTACA sites with Bsp1407I (BsrGI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: BsrGI, BstAUI). BSP1407I (BSRGI) 10U/UL 300U

Thermo Scientific™ T4 RNA-Ligase

Catalyze the ATP-dependent formation of phosphodiester bonds between 5'-P and 3'-OH termini of oligonucleotides, single-stranded RNA and DNA. T4 RNA LIGASE 10U/UL 1000U

Thermo Scientific™ FastDigest AluI

Cut at AG^CT sites with Thermo Scientific™ FastDigest™ AluI, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: AluBI). FASTDIGEST ALUI 100UL