Enzyme und Inhibitoren

Thermo Scientific™ T4 DNA-Ligase

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. T4 DNA LIGASE 5U/UL 200U

Thermo Scientific™ DNase I, RNase-frei

An endonuclease that nonspecifically cleaves single- and double-stranded DNA to release di-, tri-, and oligonucleotides with 5'-phosphate and 3'-OH groups. DNASE I RNASE-FRE 50U/UL 1000U

Thermo Scientific™ T4 Polynukleotid-Kinase (T4 PNK)

T4 Polynucleotide Kinase (T4 PNK) catalyzes the transfer of the gamma-phosphate from ATP to the 5'-OH group of oligonucleotides, ss and dsDNA and RNA. T4 PNK 2500U 10U/UL

Invitrogen™ T4 DNA Ligase Buffer

T4 DNA Ligase buffer optimizes ligation, which can be performed in 5 minutes T4 DNA Ligase Buffer, 2 x 1 ml

Thermo Scientific™ FastDigest BsmBI (Esp3I)

Cut at CGTCTC(1/5)^ sites with Thermo Scientific™ FastDigest™ BsmBI (Esp3I), which peforms best at 37°C in 5-15 minutes using universal FastDigest Buffer. FASTDIGEST BSMBI (ESP3I) 20UL

Thermo Scientific™ MspI (HpaII)

The MspI (HpaII) restriction enzyme recognizes C^CGG sites and cuts best at 37°C in Tango buffer. MSPI (HPAII) 10U/UL 5X3000U

Invitrogen™ TURBO DNA-free Kit

Ideal tool for clearing residual DNA Ambion® TURBO DNA-free, 50 reactions

Thermo Scientific™ FastDigest™ SacI

The FastDigest SacI restriction enzyme recognizes GAGCT^C sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: Eco53kI, EcoICRI, Psp124BI, SstI) FASTDIGEST SACI 200UL

Invitrogen™ DNA-free™ DNA Removal Kit

Safely eliminate DNA contamination from RNA samples DNA-free with Manual, 50 reactions

Thermo Scientific™ FastDigest BbsI (BpiI)

Cut at GAAGAC(2/6)^ sites with Thermo Scientific™ FastDigest™ BbsI (BpiI), which performs best using universal FastDigest Buffer (Isoschizomers: BpuAI, BstV2I). FASTDIGEST BBSI (BPII) 20UL

Thermo Scientific™ CpG Methyltransferase (M.SssI)

Get fast reaction times without compromising reaction efficiency with Thermo Scientific™ CpG Methyltransferase (M.SssI), one of the basic tools used in epigenetic studies. CpG Methyltransferase (M.SssI), 25 rxns

Thermo Scientific™ AatII

Cut at GACGT^C sites with AatII restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: ZraI). AATII 10U/UL 1500U

Invitrogen™ Clonase™ Gateway™ LR Clonase II Enzyme Mix

Enzymes and buffer in single mix Gateway LR Clonase II Enzyme Mix, 100 reactions

Thermo Scientific™ FastDigest MluI

Perform single-, double- or multiple DNA digestion within 5-15 minutes withthis advanced line of restriction enzymes, 100% active in one universal buffer. FASTDIGEST MLUI 100UL

Thermo Scientific™ RNase A, DNase- und proteasefrei

Specifically degrade single-stranded RNA at C and U residues with the endoribonuclease RNase A. RNASE A DNASE FRE 10MG/ML 10MG

Thermo Scientific™ FastDigest™ NcoI

The FastDigest NcoI restriction enzyme recognizes C^CATGG sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: Bsp19I). FASTDIGEST NCOI 300UL

Invitrogen™ RNase I (cloned) 100U/μL

Efficiently cleaves after all four bases of single-stranded RNA, in contrast to RNase A, which only cleaves after C and U residues RNase I (Cloned) 100 U/µl, 25000 units

Thermo Scientific™ FastDigest™ ClaI (Bsu15I)

Cut at AT^CGAT sites with ClaI (Bsu15I) restriction enzyme, which performs best at 37°C in 5-15 minutes using universal FastDigest™ Buffer. FASTDIGEST CLAI (BSU15I) 100UL

Thermo Scientific™ NdeI

The NdeI restriction enzyme recognizes CA^TATG sites and cuts best at 37°C in O buffer (Isoschizomers: FauNDI). NDEI 10U/UL 4000U

Thermo Scientific™ 10x Reaktionspuffer mit MgCl2 für DNase I

Optimize reaction condions for RNase-free DNase I with this 10X reaction Tris-HCl (pH 7.5) buffer containing MgCl2. 10X DNASE I RNASE-FREE 1ML

Thermo Scientific™ BamHI

The BamHI restriction enzyme recognizes G^GATCC sites and cuts best at 37°C in its own unique buffer. BAMHI 50U/UL 20000U

Thermo Scientific™ FastDigest SmaI

The FastDigest SmaI restriction enzyme recognizes CCC^GGG sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: TspMI, XmaCI; neoschizomer: XmaI) FASTDIGEST SMAI 200UL

Invitrogen™ CIAP (Calf Intestinal Alkaline Phosphatase)

Phosphomonoesterase that removes 3' and 5' phosphates from DNA and RNA Calf Intestinal Alkaline Phosphatase, 1000 units(20 units/ µl)

Thermo Scientific™ FastDigest EcoO109I

Cut at RG^GNCCY sites with Thermo Scientific™ FastDigest™ EcoO109I, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: DraII). FASTDIGEST ECOO109I 200UL

Invitrogen™ TURBO™ DNase (2U/μL)

Efficiently degrades and digests DNA TURBO DNase (2 U/µl), 5000 units

MP Biomedicals™ Collagenase Type I from Clostridium histolyticum

For the preparation of isolated cell suspensions 500MG COLLAGENASE

Thermo Scientific™ FastAP Wärmeempfindliche alkalische Phosphatase

Catalyze the removal of 5'- and 3'-phosphate groups from DNA, RNA, nucleotides, and proteins using this thermosensitive alkaline phosphatase . FASTAP 5x1000U 1U/UL

Thermo Scientific™ FastDigest SanDI (KflI)

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer. FASTDIGEST SANDI (KFLI) 20UL

Thermo Scientific™ HpaII

The HpaII restriction enzyme recognizes C^CGG sites and cuts best at 37°C in Tango buffer. HPAII 10U/UL 1000U

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