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  5. Invitrogen™ T4 DNA Ligase (1 U/μL) 

Invitrogen™ T4 DNA Ligase (1 U/μL)

Product Code. 10124752
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Quantity:
100 U
4 x 500 U
500 U
Unit Size:
Each
Pack of 4
This item is not returnable. View return policy

For Research Use Only. All usage must comply with product instructions.

Product Code. 10124752

Brand: Invitrogen™ 15224017

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This item is not returnable. View return policy

For Research Use Only. All usage must comply with product instructions.

Description

Includes

Vial of 5X reaction buffer (250mM Tris-HCl (pH 7.6), 50mM MgCl2, 5mM ATP, 5mM DTT, 25% (w/v) polyethylene glycol-8000)

Ideal for cloning (blunt-end or cohesive-end ligation) and adding linkers or adapters to blunt-ended DNA

T4 DNA Ligase catalyzes the formation of phosphodiester bonds in the presence of ATP between double-stranded DNAs with 3'hydroxyl and 5'phosphate termini. Single-stranded nucleic acids are not substrates for this enzyme.

  • Optimizes ligation in 5 minutes
  • Source: Purified from E. coli lambda lysogen NM989
  • Performance and quality testing: Endodeoxyribonuclease, 3' and 5' exodeoxyribonuclease assays; ligation efficiency tested
  • Unit definition: One unit catalyzes exchange of 1nmol 32P-labeled pyrophosphate into ATP in 20 min. at 37°C. One unit is equal to approximately 300 cohesive-end ligation units
  • Unit reaction conditions: 66mM Tris-HCl (pH 7.6), 6.6mM MgCl2, 10mM DTT, 66μM ATP, 3.3μM 32P-labeled pyrophosphate, and enzyme in 0.1mL for 20 min. at 37°C

ChIP-on-Chip, Chromatin Biology, Cloning, RNAi, Epigenetics & Noncoding RNA Research, Restriction Enzyme Cloning

Order Info

Shipping Conditions: Dry ice

TRUSTED_SUSTAINABILITY

Specifications

Concentration 1 U/μL
Content And Storage T4 DNA Ligase is supplied with a vial of 5X reaction buffer [250 mM Tris-HCl (pH 7.6), 50 mM MgCl2, 5 mM ATP, 5 mM DTT, 25% (w/v) polyethylene glycol-8000]. Store at -20°C.
Shipping Condition Dry Ice
Enzyme Ligase
Compatible Buffer DNA Ligase Buffer, 5X Reaction Buffer
Quantity 100 U
Product Type T4 DNA Ligase

For Research Use Only. Not for use in diagnostic procedures.

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