Cell Based Assays
Invitrogen™ Calcein AM, Cell-permeant Green and Blue Dyes
Calcein AM is a cell-permeant dye that can be used to determine cell viability in most eukaryotic cells. In live cells, the non-fluorescent Calcein AM is converted to green or blue fluorescent Calcein, after acetoxymethyl ester hydrolysis by intracellular esterases.
Molecular Probes™ LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit, for 405 nm excitation
The LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit is used to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining, or prior to elimination of biohazardous materials using formaldehyde fixation.
Invitrogen™ SYTO™ Green Fluorescent Nucleic Acid Stains
SYTO Green Fluorescent Nucleic Acid Stains exhibit bright, green fluorescence upon binding to nucleic acids. These dyes are cell-permeant and can be used to stain RNA and DNA in live cells, dead cells, and bacteria.
Invitrogen™ PrestoBlue™ and PrestoBlue™ HS Cell Viability Reagents
Monitor cell proliferation and viability with the resazurin-based PrestoBlue and PrestoBlue HS cell viability reagents. These nontoxic and cell permeable reagents quantitatively measure cell viability in as little as 10 minutes using absorbance- or fluorescence-based microplate readers.
Invitrogen™ CellMask™ Plasma Membrane Stains
Invitrogen CellMask™ plasma membrane stains enable fast and uniform labeling of the plasma membrane in almost all cell types. These stains work rapidly and can be detected using standard microscope filter sets with any imaging instrument.
Invitrogen™ LIVE/DEAD™ Cell Imaging Kit (488/570)
Sensitive two-color fluorescence cell viability assay optimized for FITC and Texas Red™ filters
Invitrogen™ Oxidized Low-density Lipoprotein (OxLDL) and DiI-OxLDL
Study lipid transport and atherosclerosis with oxidized LDL (OxLDL) and the fluorescently labeled DiI-OxLDL. Oxidized forms of human low-density lipoprotein (LDL) are used to examine scavenger receptor-mediated endocytosis of OxLDL by macrophages and endothelial cells.
Invitrogen™ CyQUANT™ LDH Cytotoxicity Assays
CyQUANT cytotoxicity assays measure cellular toxicity based on the release of cytosolic enzymes (LDH or G6PD) from damaged cells. These cytotoxicity assays utilize either colorimetric or fluorescence detection and allow continuous monitoring of cell death using microplate readers.
Invitrogen™ Click-iT™ EdU Cell Proliferation Kit for Imaging, Alexa Fluor™ 647 dye
Works the first time, every time, in less time
Invitrogen™ CellROX™ Deep Red Reagent, for oxidative stress detection
CellROX™ Deep Red Reagent is a novel fluorogenic probe for measuring cellular oxidative stress in both live and fixed cell imaging, with absorption/emission maxima at ∼644/665 nm.
Invitrogen™ CellROX™ Green Reagent, for oxidative stress detection
Novel fluorogenic probe for measuring oxidative stress in live cells
Invitrogen™ MitoTracker™ Dyes for Mitochondria Labeling
These mitochondria dyes are specially packaged in vials of 50 μg each to create freshly reconstituted dyes without the impact of freeze-thaw cycles to aid in a simplified sample prep for cell analysis workflow.
Molecular Probes™ Fluo-4, AM, cell permeant
Fluo-4 is an analog of fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels.
Invitrogen™ Amplex™ Red and Amplex™ UltraRed Reagents
The Amplex Red and UltraRed reagents are fluorogenic substrates for peroxidase. These highly sensitive and stable probes react with H2O2 in the presence of HRP for solution-based assays and ELISAs. Amplex UltraRed is a brighter version of Amplex Red with an improved pH range and sensitivity.
Invitrogen™ MitoSOX™ Mitochondrial Superoxide Indicators, for live-cell imaging
MitoSOX superoxide indicators are novel fluorogenic dyes specifically targeted to mitochondria in live cells. Oxidation of the MitoSOX reagent by mitochondrial superoxide produces bright green or red fluorescence.