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DNA Sequencing

Chemical reagents, kits, and other products used for DNA sequencing; includes sequencing and pre-sequencing kits, purification kits, buffers, standards, etc.
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1
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Applied Biosystems™ BigDye™ Terminator v1.1 Cycle Sequencing Kit

This BigDye™ Terminator v1.1 Cycle Sequencing Kit is designed for specialty applications that require optimal basecalling adjacent to the primer and for sequencing short PCR product templates with rapid electrophoresis run modules.

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Product Type Sequencing Buffer
For Use With (Equipment) SeqStudio™ Genetic Analyzer, SeqStudio™ Flex Series Genetic Analyzer, 3500/3500xL Genetic Analyzer, 3730/3730xl DNA Analyzer Thermal Cyclers: GeneAmp™ PCR System 9700 Dual Series, Veriti™ Fast 96‐Well and 384‐Well Thermal Cyclers
Technique Fluorescent Dye Terminator Sequencing
Template Compatibility BAC DNA, Plasmid DNA (≤15 Kb), PCR Amplicons, Single Stranded DNA
Product Line BigDye™ Terminator
2

Thermo Scientific™ Jurkat Genomic DNA

Use this pure human genomic DNA as a control in DNA methylation analysis, genomic DNA library construction, PCR, and Southern blotting.

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3
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Applied Biosystems™ BigDye™ Terminator v3.1 Cycle Sequencing Kit

The BigDye™ Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, plasmid, fosmid, and BAC templates.

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Format 12-well Strips,8-well Strip,96- or 384-well Plate
Product Type Sequencing Buffer
For Use With (Equipment) SeqStudio™ Genetic Analyzer, SeqStudio™ Flex Series Genetic Analyzer, 3500/3500xL Genetic Analyzer, 3730/3730xl DNA Analyzer Thermal Cyclers: GeneAmp™ PCR System 9700 Dual Series, Veriti™ Fast 96‐well and 384‐well Thermal Cyclers
Technique Fluorescent Dye Terminator Sequencing
Product Line BigDye™ Terminator
4
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Applied Biosystems™ BigDye™ Direct Cycle Sequencing Kit

The BigDye™ Direct Cycle Sequencing Kit, part of the BigDye™ product family, is a complete kit for your PCR and Sequencing needs.

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Shipping Condition Dry Ice
Format 8-well Strip,96-well Plate
Product Type Direct Cycle Sequencing Kit
Technique Fluorescent Dye Terminator Sequencing
For Use With (Application) Comparative Sequencing (Germline Mutations - 30:70 Heterozygotes), Comparative Sequencing (Germline Mutations - 50:50), GC-Rich Sequencing (>65%), GT-Rich or Difficult Template Sequencing, Standard Sequencing, Gap Closure, Homopolymer A or T, AT-Rich Sequencing (>65%), Comparative Sequencing (Germline Mutations -10:90 Heterozygotes)
Template Compatibility BAC DNA, Cosmids, Lambda DNA, PCR Amplicons (10:90 Heterozygotes), Plasmid DNA (≤15Kb), Genomic DNA (Bacterial), Fosmids, PCR Amplicons (50:50 Heterozygotes), PCR Amplicons (30:70 Heterozygotes)
Product Line BigDye
5
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Applied Biosystems™ Sanger Sequencing Kit

Sanger Sequencing Kit includes all reagents needed for sample preparation for sanger sequencing

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6

Applied Biosystems™ 3730xl DNA Analyzer 96-Capillary Array, 50 cm

This is a capillary array for the Applied Biosystems™ 3730XL DNA analyzer.

7
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Invitrogen™ Collibri™ DNA Library Preparation Kit Modules

The Invitrogen Collibri DNA Library Preparation Kit Modules are designed for a custom build of next-generation sequencing (NGS) workflow on Illumina systems.

Format Kit
For Use With (Application) DNA Sequencing; Whole-Genome Sequencing, Amplicon Sequencing
Sequencing Type Genome and DNA Sequencing
Product Line Collibri
8
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Applied Biosystems™ BDT/POP/Buffer Bundle

The BDT/POP/Buffer Bundle includes a BigDye Terminator 3.1 Cycle Sequencing Kit, POP-7 Polymer, Anode Buffer Containers, and Cathode Buffer Containers for your 3500 or SeqStudio Flex genetic analyzer. All products are offered in a single, easy-to-order bundle to provide key reagents for Sanger sequencing workflows.

Format Kit
Product Type Sequencing Buffer
Enzyme Proprietary
Control Type Plasmid DNA
Sample Type Purified DNA
Isolation Technology Enzymatic, Magnetic Bead
Label or Dye Various
For Use With (Application) Standard Sequencing
Species Various
Sequencing Type Genome and DNA Sequencing
Detection Method Fluorescent Dye
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Invitrogen™ Collibri™ ES DNA Library Prep Kits for Illumina Systems

The Invitrogen Collibri ES DNA Library Preparation kits are designed for next-generation sequencing (NGS) on Illumina systems. The kit supports whole-genome sequencing (WGS) applications and is suitable for all genomes.

Workflow Step Library Generation
Libraries Fragment Library
For Use With (Application) Whole Genome Sequencing, Resequencing, De Novo Assembly
Sequencing Type Genome and DNA Sequencing
Product Line Collibri
10
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TaqMan™ Ion Torrent™ Ion Library TaqMan™ Quantitation Kit

An easy-to-use, flexible solution for the accurate quantitation of library input for the Ion Torrent semiconductor sequencing workflow

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Invitrogen™ Collibri™ Library Quantification Kit

Invitrogen™ Collibri™ Library Quantification Kit includes a ready-to-use master mix optimized for Illumina NGS library quantification and a library dilution buffer

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Shipping Condition Dry Ice
Polymerase Platinum™ II Taq Hot Start DNA Polymerase
GC-Rich PCR Performance Low
Content And Storage Store at -20°C.
Format Kit
Product Type Library Quantification Kit
For Use With (Equipment) 7500 Fast System, 7900HT Fast System, QuantStudio™, ViiA™ 7 System, StepOne™ System, StepOnePlus™ System
Sample Type DNA,RNA
PCR Method qPCR
For Use With (Application) Illumina NGS Library Quantification
Test Time Standard
Product Line Collibri
Detection Method SYBR
12

Applied Biosystems™ GeneScan™ 1000 ROX™ dye Size Standard

The GeneScan™ 1000 ROX™ Size Standard is an internal lane size standard developed for use with Applied Biosystems™ fluorescence-based DNA electrophoresis systems.

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13

Applied Biosystems™ VetMAX™ Xeno™ Internal Positive Control DNA

VetMAX™ Xeno™ Internal Positive Control (IPC) DNA serves as an internal positive control for the DNA purification process and helps monitor for the presence of PCR inhibitors in animal health molecular detection workflows. This is of particular importance when working with sample types that have a high level of inhibitors such as feces.

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Shipping Condition Dry Ice
Includes Internal Positive Control
Format Kit
Product Type Internal Positive Control DNA
For Use With (Application) Detection and Identification of Animal Disease
Species Not Species-Specific
Product Line VetMAX, Xeno
14

Applied Biosystems™ POP-7™ Polymer for 3730/3730xl DNA Analyzers

POP-7™ Polymer is a separation matrix for the 3730 and 3730xl DNA Analyzers. POP-7™ Polymer is customized for DNA sequencing and fragment analysis.

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Accuracy 98.5% up to 900 bases (for 50 cm capillary), 98.5% up to 700 bases (for 36 cm capillary)
Product Type Polymer
For Use With (Application) Standard Sequencing, Fragment Analysis (Denaturing)
Test Time 1 hr for 36 cm capillary (std. seq.), 2 hrs for 50 cm capillary (long read seq.)
Product Line POP-7
15
Promotions Available

Applied Biosystems™ 3500xL Dx Genetic Analyzer 24-Capillary Array, 50 cm

A radio frequency identification tag is built into the frame for tracking critical run information, which is then reported on the software dashboard.

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