Invitrogen™ ZOOM™ IEF Fractionator Combo Kit

The ZOOM IEF Fractionator enriches low abundance proteins and increases the dynamic range of detection. Solution phase isoelectric focusing with the ZOOM IEF Fractionator provides reproducible separations in three hours. Fractionated samples are ready for further analysis by two-dimensional gel electrophoresis (2DE), one-dimensional gel electrophoresis (1DE), or two-dimensional liquid chromatography/mass spectrometry (2D LC/MS).

ZOOM IEF Fractionator

• Built for safety, reliability, and durability
• Includes easy-to-assemble sample chambers, in-built buffer chambers, buffer trough, removable electrode assembly, and safety lid

• Precast polyacrylamide gels that eliminate the need for manual preparation and minimize any chance of cross contamination
• These immobilized buffered disks are prelabeled, disposable, and designed for single use, ensuring consistent and reproducible fractionation
• They resolve samples into as many as seven fractions (using eight disks of specific pH) to six fractions (using six disks of specific pH) from pH 3-12; simply place each disk between the sample chambers in the ZOOM IEF Fractionator to allow separation in a specific pH range

• Proteomic grade reagents, including ZOOM 2D Protein Solubilizers urea, thiourea, CHAPS, and ampholytes, that ensure optimal conditions for sample preparation

Sample Chamber and Fractionator Unit Set-up Procedure

• The sample chamber and components fit together easily and offer a leak-proof seal
• Simply place disks between the sample chambers in the ZOOM IEF Fractionator to allow separation in a specific pH range; for example, to fractionate proteins between pH 4.6 and 5.4, flank a sample chamber with a ZOOM Disk of pH 4.6 and one of pH 5.4

• Following fractionation, the separated samples can be subjected to further analysis using narrow or broad range ZOOM Strips on the ZOOM IPGRunner™ System
• Follow with second dimension analysis using neutral pH NuPAGE™ ZOOM Gels

Applications:

Isoelectric Focusing, Protein Gel Electrophoresis, Protein Sample Fractionation, Protein Sample Preparation and Protein Purification, Proteins, Expression, Isolation and Analysis