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  6. PeproTech VEGF-165 Polyclonal Antibody, PeproTech®, Invitrogen™

PeproTech VEGF-165 Polyclonal Antibody, PeproTech®, Invitrogen™
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Product Code. 17884183
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50 μg
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50µg
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17884183 50 μg 50µg
17864143 100 μg 100µg
17804763 1 mg 1mg
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Product Code. 17884183 Supplier PeproTech Supplier No. 500P10G50UG

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Description

Goat Polyclonal Antibody

AA Sequence of recombinant protein: APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR. Preparation: Produced from sera of goats immunized with highly pure Recombinant Human VEGF 165. Anti-Human VEGF 165-specific antibody was purified by affinity chromatography employing an immobilized Human VEGF 165 matrix. Sandwich ELISA: To detect Human VEGF 165 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human VEGF 165 (500-P10GBt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human VEGF 165. Western Blot: To detect Human VEGF 165 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human VEGF 165 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

The vascular endothelial growth factor (VEGF) family currently includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. VEGF and its receptor system have been shown to be the fundamental regulators in the cell signaling of angiogenesis. Most tumors have the absolute requirement of angiogenesis and VEGF has been described as the most potent angiogenic cytokine linked to this process. To date 5 different isoforms of VEGF have been described. These isoforms are generated as the result of alternative splicing from a single VEGF gene. These various isoforms have been shown to bind to two tyrosine-kinase receptors flt-1 (VEGFR-1) and flk-1/KDR (VEGFR-2), which have been found to be expressed almost exclusively on endothelial cells.
TRUSTED_SUSTAINABILITY

Specifications

Antigen VEGF-165
Applications ELISA, Western Blot
Classification Polyclonal
Concentration 0.1-1.0 mg/mL
Conjugate Unconjugated
Formulation PBS with no preservative
Gene Vegfa
Gene Accession No. P15692
Gene Alias H-VEGF-165; MVCD1; M-VEGF-165; vascular endothelial growth factor 165b; vascular endothelial growth factor A; vascular endothelial growth factor A121; vascular endothelial growth factor A165; vascular permeability factor; VEGF; VEGF164; VEGF165; Vegfa; VEGF-A; VPF
Gene Symbols Vegfa
Host Species Goat
Immunogen E.coli-derived Recombinant Human VEGF 165.
Purification Method Antigen affinity chromatography
Quantity 50 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 7422
Target Species Human
Content And Storage -20°C
Product Type Antibody
Form Lyophilized
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