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  5. Thermo Scientific™ Tryptone Bile X-Glucuronide (TBX) Medium

Thermo Scientific™ Tryptone Bile X-Glucuronide (TBX) Medium

Product Code. 11974162
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Quantity:
10/Pk.
Fill Weight:
17g ± 5%
Media Type:
TBX Medium
Unit Size:
Pack of 10
This item is not returnable. View return policy
This item is not returnable. View return policy

Description

Detect and enumerate Escherichia coli in food and animal feed samples with this selective, chromogenic, ready-to-use medium.

Detect and enumerate Escherichia coli in food samples with Thermo Scientific™ Typtone Bile X-Glucuronide (TBX) Medium. TBX Medium builds on the speed, reliability and recovery from frozen samples and detection of poor lactose fermenters offered by the original Tryptone Bile Agar1,2, with the addition of a chromogenic agent, X-glucuronide, which detects glucuronidase activity: an enzyme shown to be highly specific for Escherichia coli3. The released chromophore in TBX Medium is insoluble and accumulates within the cell, making colored target colonies easy to identify

Most Escherichia coli strains can be differentiated from other coliforms by the presence of the enzyme glucuronidase. However, approximately 3 to 4% of Escherichia coli are glucuronidase negative, notably Escherichia coli O157 strains4.

The chromogen in TBX Medium is 5-bromo-4-chloro-3-indolyl-beta-D-glucuronide (X-glucuronide) and is targeted by glucuronidase. Escherichia coli cells are able to absorb this complex intact and intracellular glucuronidase splits the bond between the chromophore and the glucuronide. The released chromophore is colored and builds up within the cells, causing Escherichia coli colonies to be colored blue/green.

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Remel™ and Oxoid™ products are now part of the Thermo Scientific brand, combining powerful manual, semi-automated and fully automated test products and a comprehensive line of media and diagnostic products to offer a complete, end-to-end solution to quickly deliver the products you need and the quality results your laboratory depends on.

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Specifications

Color Ivory, transparent
Content And Storage 2° to 12°C, in the dark
Format 90mm Monoplate
Manufacturing Origin Germany
Media Type TBX Medium
Product Type Agar
Fill Volume 17 mL
Fill Weight 17g ± 5%
Packaging Type Flow Wrapped - Single Layer
Quantity 10/Pk.
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