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THP-1 (human acute monocytic leukemia) Whole cell lysate, Denatured; Abnova
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Quantity:
200 μg
Unit Size:
200µg
Beschreibung
Western Blotting
Spezifikation
Spezifikation
| For Use With (Application) | Western Blot |
| Tissue | Blood, peripheral |
| Description | Whole cell lysate (denatured) |
| Lysis Buffer | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. |
| Preparation Method | Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was mixed in 5X Sample Buffer to become final 1X Sample Buffer as Storage Buffer. The lysate was heated at 95°C for 5 min, and cooled rapidly. |
| Quality Control Testing | 12.5% SDS-PAGE Stained with Coomassie Blue. |
| Quantity | 200 μg |
| Storage Buffer | In ready-to-use 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue). |
| Host Species | Human |
| Concentration | 3 mg/mL |
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