Thermo Scientific™ Phusion U Hot Start PCR Master Mix

Phusion U DNA Polymerase is a novel engineered high-fidelity enzyme developed using fusion technology. Designed with a proprietary mutation in the dUTP binding pocket of Phusion polymerase, Phusion U polymerase is able to overcome important limitations of proofreading enzymes to incorporate dUTP and read through uracil present in DNA templates.

Phusion U polymerase features properties of Phusion DNA polymerases and offers the ability to amplify long amplicons up to 20 kb and a high specificity with affibody based hot start.

These features make Phusion U Hot Start DNA Polymerase an excellent choice for such applications as amplification of bisulfite-converted or damaged DNA as well as use of carryover contamination control.

Highlights

• High-fidelity DNA polymerase (25X Taq)
• Engineered to incorporate dUTP and amplify uracil containing templates
• Hot start for reduced nonspecific amplification and primer degradation
• Only template and primers need to be added for use
• Short extension times (15–30 s/kb)

Applications

• Amplification of bisulfite-converted DNA
• Amplification of damaged or aged DNA
• Carry-over contamination control
• Uracil-excision based (USER) cloning methods

Using Phusion DNA Polymerases

• Annealing rules for Phusion DNA polymerases are different from many common DNA polymerases (such as Taq DNA polymerases).