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  7. Thermo Scientific™ Phire Tissue Direct PCR Master Mix

Thermo Scientific™ Phire Tissue Direct PCR Master Mix

Product Code. 15252606 Shop All Thermo Scientific Products
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No. of Reactions:
1250 Reactions
250 Reactions
Unit Size:
250 reactions
500 reactions
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Product Code. 15252606

Brand: Thermo Scientific™ F170L

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620.10 EUR valid until 2025-12-19
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This item is not returnable. View return policy

Description

Thermo Scientific Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, human, fish, birds and insects.

Thermo Scientific Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, humans, fish, birds, and insects. The Master Mix containing Phire Hot Start II DNA Polymerase is specially formulated to perform PCR in the presence of different animal tissue–derived inhibitors such as collagen, melanin and eumelanin (hair, skin), and myoglobin (muscle). The kit also includes DNARelease Additive, which can be used to improve the release of DNA from difficult tissues.

Features

  • Direct PCR reduces time-consuming and expensive DNA purification steps
  • Available Dilution & Storage protocol allows multiple PCR reactions from a single sample
  • Specially engineered Phire DNA polymerase for extremely short PCR protocol times
  • Master mix format with premixed gel loading dye minimizes cross-contamination, reduces sample handling, and allows direct loading on gel

Applications

  • Genotyping
  • Transgene detection
  • Knockout analysis
  • Sequencing

Notes

  • The optimal annealing temperature for Phire DNA Polymerases may differ significantly from that of Taq-based polymerases.
TRUSTED_SUSTAINABILITY

Specifications

Concentration 2X
Content And Storage • 2X Phire Tissue Direct PCR Master Mix, 10 x 1.25 mL
• Dilution Buffer, 2 x 5 mL
• DNARelease Additive, 1.3 mL
• 25 μM Control Primer Mix, 40 μL
• Nuclease-free water, 10 x 1.25 mL
• 50 μg O'GeneRuler Express DNA Ladder

Store at -20°C.
GC-Rich PCR Performance Medium
PCR Method Direct PCR
Polymerase Phire Hot Start II DNA Polymerase
Reaction Speed Fast
Includes Label or Dye Yes
Product Type Direct PCR Master Mix
Quantity 1,250 reactions
Shipping Condition Wet or Dry Ice
For Use With (Application) Genotyping, Hot-start PCR
Hot Start Built-In Hot Start
No. of Reactions 1250 Reactions
Overhang Blunt
Reaction Format SuperMix or Master Mix
Sample Type Tissue
Starting Material DNA
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For Research Use Only. Not for use in diagnostic procedures.

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