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Description
- The Applied Biosystems™ TaqMan™ RNaseP Assay with ABY™ dye-labeled QSY™ probe provides a pre-formulated assay for quantitating human RNase P. This assay enables relative gene expression quantification in cDNA samples when used with other gene expression assays.
- It consists of an ABY™ dye-labeled QSY™ probe plus sequence-specific forward and reverse primers, and it can be used for multiplex or singleplex PCR reactions.
- Eliminate assay design time using pre-designed primers and pre-designed ABY™ dye-labeled TaqMan™ probe
- Minimize experimental optimization time
- Use in conjunction with FAM™ or VIC™ dye-labeled predesigned gene expression assays
- Save time in assay design, formulation, and testing by using the TaqMan™ RNase P Assay as your control when quantifying gene expression
- This pre-designed probe and primer set enables you to normalize the amount of sample RNA or DNA in a reaction when used with a control sample. Or use it for relative gene expression quantification in cDNA samples when used with other gene expression assays.
- The ABY™ dye is optimized for use with the 3rd filter of our QuantStudio™, ViiA™ 7, and 7500 series real-time PCR instruments.
Specifications
Specifications
| Accession Number | NR_002312.1 |
| Amplicon Size | 87 |
| Content And Storage | Primer-Probe Set(s) |
| Detection Method | Primer-probe |
| Format | Tube |
| PCR Method | qPCR |
| Gene Symbol | RNase P (RPPH1) |
| Internal Probe Modification | QSY™ Non-Fluorescent Quencher (3') |
| Label or Dye | ABY |
| Product Line | TaqMan |
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Frequently Asked Questions (FAQs)
ABY dye has an excitation wavelength of 568 nm and emission wavelength of 583 nm.
JUN dye has an excitation wavelength of 606 nm and emission wavelength of 618 nm.
Cynanine 5 dye has an excitation wavelength of 649 nm and emission wavelength of 667 nm.
Cyanine 5.5 dye has an excitation wavelength of 678 nm and emission wavelength of 695 nm.
Please refer to your instrument user guide for additional information on compatibility with different dye channels.
For Research Use Only. Not for use in diagnostic procedures.
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