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Gibco™ RPMI 1640 Medium, powder
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Quantity:
10 L
1 L
50 L
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1 L
10 L
50 L
Beschreibung
Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. Life Technologies offers a variety of Gibco™ RPMI 1640 modifications for a range of cell culture applications.
Gibco™ RPMI 1640 is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 contains biotin, vitamin B12, and PABA which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. Customers using Gibco™ RPMI 1640 in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from Life Technologies to reference our Type II Drug Master File (DMF).
cGMP Manufacturing and Quality System
Gibco™ RPMI 1640 is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, Life Technologies offers a comparable Gibco™ RPMI 1640 product made in our Grand Island facility (31800-089). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.
RPMI 1640 contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
Powder forms of Gibco™ L-15 cell culture medium require sodium bicarbonate supplementation pH adjustment, and filtration at the time of preparation.
Gibco™ RPMI 1640 is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 contains biotin, vitamin B12, and PABA which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. Customers using Gibco™ RPMI 1640 in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from Life Technologies to reference our Type II Drug Master File (DMF).
cGMP Manufacturing and Quality System
Gibco™ RPMI 1640 is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, Life Technologies offers a comparable Gibco™ RPMI 1640 product made in our Grand Island facility (31800-089). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.
RPMI 1640 contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
Powder forms of Gibco™ L-15 cell culture medium require sodium bicarbonate supplementation pH adjustment, and filtration at the time of preparation.
Spezifikation
Spezifikation
| Form | Powder |
| Product Type | Roswell Park Memorial Institute (RPMI) 1640 Medium |
| With Additives | Glutamine, Phenol Red |
| Without Additives | No HEPES, No Sodium Pyruvate, No Sodium Bicarbonate |
| For Use With (Application) | Dulbecco's Modified Eagle Medium |
| Quantity | 10 L |
| Certifications/Compliance | ISO 13485 standards, FDA |
| pH | 7.4 to 7.6 |
| Content And Storage | 2 to 8°C |
| Description | Roswell Park Memorial Institute (RPMI) 1640 Medium, 10L, Powder, 7.4 to 7.6 pH, With L-Glutamine, Phenol Red, 36 Months Shelf Life, 230 to 280mOsm/kg Osmolality, For Mammalian Cell Culture |
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