BD Rhapsody™ Intracellular AbSeq Buffer Kit

Each intracellular BD™ AbSeq Antibody is conjugated to an antibody-specific oligonucleotide barcode for profiling alongside surface protein and mRNA expression. This product provides reagents to preserve, permeabilize, and stain cells with intracellular BD™ AbSeq Antibody-Oligos.

Prior to beginning the intracellular CITE-seq protocol, surface antigen staining should be performed using one of the following protocols:

• BD Rhapsody™ System Single-Cell Labeling with BD™ AbSeq Ab-Oligos (1 plex to 40 plex) Protocol 23-24262 or
• BD Rhapsody™ System Single-Cell Labeling with the BD™ Single-Cell Multiplexing Kit and BD™ AbSeq Ab-Oligos 23-21339 to support single-cell labeling with BD™ AbSeq Ab-Oligos without and with sample multiplexing, respectively

These protocols have been tested for use with up to 40 surface markers. The following protocols, BD Rhapsody™ System Single-Cell Labeling with BD™ AbSeq Ab-Oligos (41 plex to 100 plex) protocol, 23-22314 or BD Rhapsody™ System Single-Cell Labeling with BD™ Single-Cell Multiplexing Kit and BD™ AbSeq Ab-Oligos protocol, 23-22354, are available for use with high-plex experiments, however, additional optimization may be required.