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R&D Systems™ Recombinant Human TNF-alpha Protein

Product Code. 18167300
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Quantity:
5 μg
Unit Size:
5µg
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Product Code. 18167300

Brand: R&D Systems™ 210TA005/CF

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This item is not returnable. View return policy

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED50 for this effect is 25-100 pg/mL. The specific activity of Recombinant Human TNF-alpha is approximately 7.6 x 104 IU/μg, which is calibrated against human TNF-alpha WHO International Standard (NIBSC code: 88/786). Specific activity is for reference purposes only and is not routinely tested.
TRUSTED_SUSTAINABILITY

Specifications

Formulation Lyophilized from a 0.2μm filtered solution in PBS.
Gene ID (Entrez) 7124
Molecular Weight (g/mol) 17.5 kDa
Name TNF-alpha
Quantity 5 μg
Source E. coli-derived human TNF-alpha protein Val77-Leu233, with and without an N-terminal Met
Endotoxin Concentration Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED50 for this effect is 25-100 pg/mL.
Gene Alias APC1 protein, Cachectin, Cachetin, DIF, TNF, TNF, monocyte-derived, TNF-a, TNFA, TNFalpha, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFG1F, TNFSF1A, TNFSF2, TNFSF2TNF superfamily, member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha
Conjugate Unconjugated
Recombinant Recombinant
Purity or Quality Grade >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
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