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enQuireBio™ Recombinant Human NAD-dependent malic enzyme, mitochondrial Protein

Product Code. 15902928
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15902928 5 μg 5µg
15992918 25 μg 25µg
15972918 100 μg 100µg
15982918 250 μg 250µg
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Product Code. 15902928 Supplier enQuireBio™ Supplier No. QP10156ec5ug

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Description

A DNA sequence encoding the Human NAD-dependent malic enzyme, mitochondrial, was expressed in the hosts and tags indicated.

Specifications

Accession Number NP_001161807.1
Gene ID (Entrez) 4200
Name NAD-dependent malic enzyme, mitochondrial Protein
Quantity 5 μg
Regulatory Status Research Use Only
Endotoxin Concentration Less than 1.0 EU/ug as determined by LAL method.
Gene Symbol ME2
Protein Length Partial (see sequence information for more details).
Biological Activity Malic Enzyme activity was assayed spectrophotometrically at 340nm as described in Mandela and Sauer (1975). The standard reaction mixture contained 50 mM Tris-HCl, 3 mM MnCl2, 5 mM malate, 0.12 mM NADP+, 2.5 mM fumarate. Assay was performed in a Beckman spectrophotometer. The Km value is 1.5 ± 0.6 mM.
Product Type Recombinant Protein
Cross Reactivity Human
Species E. coli
Protein Tag His
Sequence MLHIKEKGKP LMLNPRTNKG MAFTLQERQM LGLQGLLPPK IETQDIQALR FHRNLKKMTS PLEKYIYIM GIQERNEKLF YRILQDDIES LMPIVYTPTV GLACSQYGHI FRRPKGLFIS ISDRGHVRS IVDNWPENHV KAVVVTDGER ILGLGDLGVY GMGIPVGKLC LYTACAGIRP DRCLPVCID VGTDNIALLK DPFYMGLYQK RDRTQQYDDL IDEFMKAITD RYGRNTLIQF EDFGNHNAF RFLRKYREKY CTFNDDIQGT AAVALAGLLA AQKVISKPIS EHKILFLGAG EAALGIANL IVMSMVENGL SEQEAQKKIW MFDKYGLLVK GRKAKIDSYQ EPFTHSAPES IPDTFEDAV NILKPSTIIG VAGAGRLFTP DVIRAMASIN ERPVIFALSN PTAQAECTAE EAYTLTEGR CLFASGSPFG PVKLTDGRVF TPGQGNNVYI FPGVALAVIL CNTRHISDSV FLEAAKALT SQLTDEELAQ GRLYPPLANI QEVSINIAIK VTEYLYANKM AFRYPEPEDK AKYVKERTW RSEYDSLLPD VYEWPESASS PPVITEHHHH HH>
Buffer 0.2um filtered PBS, pH 7.4. , lyophilized
Purity or Quality Grade Greater than 95% as determined by SDS-PAGE and HPLC.
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