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R&D Systems™ Recombinant Human Active Chk1 Protein

Product Code. 18136350
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10ug, Carrier Free
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Product Code. 18136350

Brand: R&D Systems™ 1630KS010

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Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

The specific activity of Chk1 was determined to be 228 nmol/min/mg using a synthetic peptide substrate (KKKVSRSGLYRSPSMPENLNRPR).
TRUSTED_SUSTAINABILITY

Specifications

For Use With (Application) Bioactivity
Formulation Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
Molecular Weight (g/mol) Observed MW: 82kDa
Source Spodoptera frugiperda, Sf 9 (baculovirus)-derived human Chk1 protein
Storage Requirements This product is stable at ≤ -70° C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.
Conjugate Unconjugated
Buffer Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM Glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
Content And Storage This product is stable at <= -70°C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store (at recommended temperature. Avoid repeated freeze-thaw cycles.)
Purity or Quality Grade >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie™ Blue stain (at 5 μg / lane.)
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