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R&D Systems™ Recombinant E. coli BirA His-tag Protein

Product Code. 18716686
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Quantity:
100 μg
Unit Size:
100µg
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18716686 100 μg 100µg
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Product Code. 18716686 Supplier R&D Systems™ Supplier No. 10464BA100

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Description

Measured by its ability to generate pyrophosphate from the biotinylation reaction.

The pyrophosphate is subsequently hydrolyzed using Recombinant Yeast Inorganic Pyrophosphatase/PPA1 (ryPPA1) (Catalog # 8088-PP ). The specific activity is >10.0 pmol/min/μg, as measured under the described conditions.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number NP_418404.1
Formulation Supplied as a 0.2 μm filtered solution in MES, NaCl, TCEP, EDTA and Glycerol.
Gene ID (Entrez) 948469
Molecular Weight (g/mol) Observed: 30-38 kDa, under reducing conditions
Quantity 100 μg
Source E. coli-derived e. coli BirA protein Lys2-Lys321, with an N-terminal Met & 6-His tag
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after opening.
Endotoxin Concentration <0.10 EU / 1 μg of the protein by the LAL method.
Conjugate Unconjugated
Purity or Quality Grade >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Protein BirA
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