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Invitrogen™ ReadyProbes™ Tissue Autofluorescence Quenching Kit
Minimizes unwanted autofluorescence in FFPE and frozen tissues due to aldehyde fixation, red blood cells, and structural elements such as collagen and elastin
Brand: Invitrogen™ R37630
Additional Details : Weight : 0.01440kg
Description
- The ReadyProbes Tissue Autofluorescence Quenching Kit minimizes unwanted autofluorescence in FFPE and frozen tissues due to aldehyde fixation, red blood cells, and structural elements such as collagen and elastin.
- If the target signal is low enough, then this background signal can interfere with the interpretation of immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence(IF), and in situ hybridization (ISH) assays.
- The ReadyProbes Tissue Autofluorescence Quenching Kit minimizes most kinds of autofluorescence, with the exception of autofluorescence from lipofuschin granules.
- The ReadyProbes Tissue Autofluorescence Quenching Kit contains three components that are combined just before use. Combine components A, B, and C in a 1:1:1 ratio.
- Mix components A and B together before adding component C. The resulting solution is then applied to the tissue for 2–5 minutes at room temperature.
- This is done after the blocking, primary antibody, and secondary antibody steps, but before application of any counterstains like nuclear stains.
Specifications
| 1 Kit | |
| Approved for shipment at Room Temperature or on Wet Ice | |
| Blocking Reagent |
| A kit containing 5 mL each ready-to-use (1X) solutions of reagent A, B, and C in three clear HDPE bottles. |
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| ReadyProbes™ |
For Research Use Only. Not for use in diagnostic procedures.