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Invitrogen™ Rat TNF alpha ELISA Kits

Product Code. 10741585
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Quantity:
1 Plate Kit
Unit Size:
1 set
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Product Code. 10741585

Brand: Invitrogen™ ER3TNFA

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Description

Measure levels of rat tumor necrosis factor alpha (TNFa) levels in rat serum, plasma and culture supernatant samples using 96-well plates.

Assay TNFα (rat tumor necrosis factor alpha) levels in rat serum, plasma (EDTA, heparin) and culture supernatant samples with our Thermo Scientific Rat TNF alpha ELISA Kits.

These rat TNF alpha ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of tumor necrosis factor alpha (TNF-alpha, TNFα) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

  • Target: Natural and recombinant rat TNFα (rat tumor necrosis factor alpha)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 31 to 2500pg/mL

  • Sensitivity: < 15pg/mL

  • Precision: Intra-assay CV < 10%; inter-assay CV < 10%

  • Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 3 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with human TNFα, human TNFβ or porcine TNFα. Mouse TNFα cross-reacts weakly (< 3%)

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

TRUSTED_SUSTAINABILITY

Specifications

Assay Range 31 to 2500pg/mL
Assay Sensitivity <15pg/mL
Description Rat TNF alpha ELISA Kit
Immunoassay Kit Format Colorimetric ELISA (TMB substrate) with Plates
Sample Type Cell Culture Supernatant, Plasma (EDTA, Citrate, Heparin), Serum
Specificity No significant cross-reaction with human TNFα, human TNFβ or porcine TNFα. Mouse TNFα cross-reacts weakly (< 3%)
Sufficient For 96 microplate wells
Kit Contents Standard Diluent, 25mL, TMB Substrate Solution, 13mL, Stop Solution, 13mL, Pre-Treatment Buffer, 6mL, Lyophilized Recombinant Rat TNF alpha Standard, 2 vials, Biotinylated Antibody Reagent, 6mL, Streptavidin-HRP Reagent, 14mL, Anti-Rat TNF alpha Precoated 96-well Strip Plate, 1 ea, 30X Wash Buffer, 50mL
Measurement Absorbance at 450nm minus absorbance at 550nm
Precision Intra-assay CV <10%; inter-assay CV <10%
Quantity 1 Plate Kit
Sample Volume 50 μL
Target Natural and recombinant rat TNFa (rat tumor necrosis factor alpha)
Total Assay Time 3 hr.
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