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Proteintech™ Rat TGF-beta1 ELISA Kit

Artikelnummer. 16811656 Alle ansehen Proteintech Produkte
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Target Species:
Rat
Packungsgröße:
96 tests
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16811656 Rat 96 tests
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Artikelnummer. 16811656 Lieferant Proteintech™ Lieferanten-Nr. KE2001096T

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Beschreibung

KE20010 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The TGF-beta1 ELISA kit is to be used to detect and quantify protein levels of endogenous TGF-beta1.

  • The assay recognizes Rat TGF-beta1. An antibody specific for TGF-beta1 has been pre-coated onto the microwells.
  • The TGF-beta1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for TGF-beta1 is added to detect the captured TGF-beta1 protein.
  • For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
  • TGF-beta is a member of the transforming growth factor beta (TGFB) family of cytokines, which are multifunctional peptides that regulate proliferation, differentiation, adhesion, migration, and other functions in many cell types.
  • TGF-beta is produced by a number of cell types including regulatory T cells, fibroblasts, epithelial cells, and endothelial cells.
  • TGF-beta acts synergistically with TGFA in inducing transformation. It also acts as a negative autocrine growth factor
  • TGF-beta plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
  • TGF-beta appears to promote late stage progression and metastasis in some cancers.

Spezifikation

Accession Number BC076380
Assay Range 31.25 to 2000 pg/mL
Assay Sensitivity 3.4 pg/mL
Certifications/Compliance ISO 13485
Detection Method Sandwich ELISA, Double Antibody
Immunoassay Kit Format Coated ELISA Kit
Sample Type Plasma, Serum
Substrate DAB
Target Species Rat
Gene ID (Entrez) 59086
Host Species Rat
Immunoassay Kit Grade Solid
Interassay CV <10%
Intraassay CV <10%
Shipping Condition Blue ice
Storage Requirements -20°C to +4°C
UniProt ID P17246
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Sicherheit und Handhabung

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Produktidentifikator
  • 96TEST Human GRP ELISA Kit
 Signalwort
  • Warning
 Gefahrenkategorie
  • Serious eye damage/eye irritation Category 1
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2
  • Specific target organ toxicity Category 3
 Gefahrenhinweis
  • H302-Harmful if swallowed.
  • H315-Causes skin irritation.
  • H318-Causes serious eye damage.
  • H319-Causes serious eye irritation.
  • H332-Harmful if inhaled.
  • H335-May cause respiratory irritation.
 Sicherheitshinweis
  • P201-Obtain special instructions before use.
  • P202-Do not handle until all safety precautions have been read and understood.
  • P234-Keep only in original container.
  • P264-Wash skin thoroughly after handling.
  • P270-Do not eat, drink or smoke when using this product.
  • P273-Avoid release to the environment.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
  • P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
  • P302+P352-IF ON SKIN: Wash with plenty of water/soap.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P308+P313-IF exposed or concerned: Get medical advice/attention.
  • P330-Rinse mouth.
  • P362-Take off contaminated clothing.
  • P405-Store locked up.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Ergänzende Informationen
  • MIXTURE LIST-Contain: Sulphuric Acid, Tetramethylbenzidine
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