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Proteintech™ Rat Insulin ELISA Kit

Product Code. 16891646 Shop All Proteintech Products
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Target Species:
Rat
Unit Size:
96 tests
Product Code. Target Species unitSize
16891646 Rat 96 tests
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Product Code. 16891646

Brand: Proteintech™ KE2000896T

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This item is not returnable. View return policy

Description

KE20008 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Insulin ELISA kit is to be used to detect and quantify protein levels of endogenous Insulin.

  • The assay recognizes Rat Insulin. An antibody specific for Insulin has been pre-coated onto the microwells.
  • The Insulin protein in samples is captured by the coated antibody after incubation.
  • Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for Insulin is added to detect the captured Insulin protein.
  • For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm
  • Insulin is a peptide hormone, produced by beta cells of the pancreas, and is central to regulating carbohydrate and fat metabolism in the body.
  • It participates in glucose utilization, protein synthesis and in the formation and storage of neutral lipids. Insulin is synthesized as a precursor molecule, proinsulin, which is processed prior to secretion.
  • A- and B-peptides are joined together by a disulfide bond to form insulin, while the central portion of the precursor molecule is cleaved and released as the C-peptide.
  • Defects in insulin results in type 1 diabetes mellitus.

Specifications

Accession Number NM_019129.3
Assay Range 0.31 to 20 ng/mL
Assay Sensitivity 0.2 ng/mL
Certifications/Compliance ISO 13485
Detection Method Sandwich ELISA, Double Antibody
Immunoassay Kit Format Coated ELISA Kit
Sample Type Plasma, Serum
Substrate DAB
Target Species Rat
Gene ID (Entrez) 24505
Host Species Rat
Immunoassay Kit Grade Solid
Interassay CV <10%
Intraassay CV <10%
Shipping Condition Blue ice
Storage Requirements -20°C to +4°C
Total Assay Time 3.5 hr.
UniProt ID P01322
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Safety and Handling

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Product Identifier
  • 96TEST Rat Insulin ELISA Kit
 Signal Word
  • Warning
 Hazard Category
  • Serious eye damage/eye irritation Category 1
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2
  • Specific target organ toxicity Category 3
 Hazard Statement
  • H302-Harmful if swallowed.
  • H315-Causes skin irritation.
  • H318-Causes serious eye damage.
  • H319-Causes serious eye irritation.
  • H332-Harmful if inhaled.
  • H335-May cause respiratory irritation.
 Precautionary Statement
  • P201-Obtain special instructions before use.
  • P202-Do not handle until all safety precautions have been read and understood.
  • P234-Keep only in original container.
  • P264-Wash skin thoroughly after handling.
  • P270-Do not eat, drink or smoke when using this product.
  • P273-Avoid release to the environment.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
  • P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
  • P302+P352-IF ON SKIN: Wash with plenty of water/soap.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P308+P313-IF exposed or concerned: Get medical advice/attention.
  • P330-Rinse mouth.
  • P362-Take off contaminated clothing.
  • P405-Store locked up.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contains : Sulphuric Acid, Tetramethylbenzidine
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