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  6. Macherey-Nagel™ Protino Ni-IDA Resin for His-tag Protein Purification

Macherey-Nagel™ Protino Ni-IDA Resin for His-tag Protein Purification

Product Code. 11912292
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Quantity:
120 g
600 g
Unit Size:
120g
600g
This item is not returnable. View return policy

Product Code. 11912292

Brand: Macherey-Nagel™ 745210.120

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This item is not returnable. View return policy

Description

The matrix of choice for highest protein purity as dry bulk material.

Protino™ Ni-IDA, the purer alternative for higher yields of His-tag proteins

  • Chelating group IDA, three protein binding sites
  • High protein yield/recovery even from diluted samples
  • High protein concentration
  • High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices
  • Dry resin, storage at room temperature

Protino™ Ni-IDA packed columns

  • Ready to use pre-packed columns filled with dry Protino™ Ni-IDA resin
  • Buffers included

Protino™ Ni-IDA resin

  • Dry bulk matrix
  • Rigid support silica matrix, compatible with high pressure and high flow rates, does not shrink or swell - highest flexibility
  • Batch procedure, gravity flow column chromatography, FPLC™ applications
  • Empty Protino™ columns available for use with Protino™ Ni-IDA resin
  • Empty 96 well receiver plates available for use with Protino™ Ni-IDA resin

Protino™ Multi-96 Ni-IDA

  • Ready to use pre-packed 96 well plates filled with dry Protino™ Ni-IDA resin
  • Buffers included

For purification of polyhistidine-tagged proteins, batch binding, gravity flow column chromatography, MPLC/FPLC™ applications.

Specifications

Bed Volume 10 mg/mL
Binding Capacity 20mg/g
Flow Rate 0.5 to 1.0 mL/min./cm2
Kit Contents Dry silica bulk resin
Matrix Macroporous silica
Content And Storage Room Temperature
Max. Operating Pressure 145 psi
Quantity 120 g
For Use With (Application) Purification of polyhistidine-tagged proteins
Type Resin

Safety and Handling

compliance-icons compliance-icons
Signal Word
  • Danger
 Hazard Category
  • Skin sensitiser Category 1
  • Respiratory sensitiser Category 1
  • Germ cell mutagenicity Category 2
  • Carcinogenicity Category 1A
  • LONG-TERM AQUATIC HAZARD Chronic 1
 Hazard Statement
  • H317-May cause an allergic skin reaction.
  • H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
  • H341-Suspected of causing genetic defects.
  • H350i-May cause cancer by inhalation.
  • H410-Very toxic to aquatic life with long lasting effects.
 Precautionary Statement
  • P201-Obtain special instructions before use.
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P273-Avoid release to the environment.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
  • P391-Collect spillage.
  • P405-Store locked up.
 Supplemental information
  • MIXTURE LIST-Contains : resin with Ni in nickel(II) complexes
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