Invitrogen™ PIR-A/B Monoclonal Antibody (10-1-PIR), APC, eBioscience™, Invitrogen™

Description

Description: This 10-1-PIR monoclonal antibody reacts with mouse paired Ig-like receptors of activating (PIR)-A and -B. These cell surface glycoproteins, which contain six extracellular Ig-like domains with distinct transmembrane and cytoplasmic regions, are expressed together on B lymphocytes, dendritic cells, macrophages, granulocytes, platelets, and mast cells. However, expression of PIR-A/B has not been observed on T cells, NK cells, and erythrocytes. The PIR-A receptor interacts with signaling molecules containing immunoreceptor tyrosine-based activation motifs (ITAMs) which lead to its activating function. In contrast, the PIR-B receptor associates with proteins possessing immunoreceptor tyrosine-based inhibitory motifs (ITIMs); therefore, this receptor has been shown to have an inhibitory function. PIRs bind MHC class I to modulate cell signaling and homeostasis of the immune system. Moreover, PIR-B knockout mice have been shown to exhibit susceptibility to Salmonella infection. Crossblocking studies indicate that 10-1-PIR recognizes a different epitope from 6C1 (cat. no. 46-5978). Applications Reported: This 10-1-PIR antibody has been reported for use in flow cytometric analysis. Applications Tested: This 10-1-PIR antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per test.

Paired Ig-like receptors of activating (PIR)-A and -B are expressed on B lymphocytes, macrophages, dendritic cells, platelets, granulocytes, and mast cells, but not on T cells, NK cells, and erythrocytes. These cell surface glycoproteins contain six extracellular Ig-like domains with distinct cytoplasmic and transmembrane regions. The PIR-A receptor activates through signaling molecules containing immunoreceptor tyrosine-based activation motifs. In contrast, the PIR-B receptor inhibits through proteins possessing immunoreceptor tyrosine-based inhibitory motifs. PIRs bind MHC class I to modulate immune system homeostasis and cell signaling.