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Description
Perform convenient removal of SDS from small-volume (0.1 to 10mL) protein samples with Thermo Scientific™ SDS-Out SDS Precipitation Reagent.
The simple reagent and kit effectively remove excess SDS from small protein samples. Sodium dodecyl sulfate (SDS) is a detergent typically used in cell lysis reagents and other protein solubilization buffers. Traditional methods for SDS removal include prolonged dialysis, anion exchange chromatography, affinity columns or and acetone precipitation. By comparison, the SDS-Out Precipitation Reagent is relatively easy to use and provides excellent protein recovery.
Highlights:
- Convenient removal of SDS from microliter-volume samples
- Use for samples containing 0.1 to 1% SDS
- Minimal dilution of small-volume samples
- Protein recovery is 90 to 100% (based on tests with BSA, cytochrome C, soybean trypsin inhibitor, ovalbumin, ribonuclease A, myoglobin and human IgG)
Includes:
Spin cup and collection tube accessories for use with the SDS-Out Reagent
Specifications
Specifications
| Product Type | SDS Precipitation Kit |
| Content And Storage | Sufficient For: Precipitation of SDS from 200 mL of protein solution • SDS Precipitation Reagent, 10 mL • Spin Cup Columns, 12 columns • Collection Tubes, 12 tubes Upon receipt, store components at 4°C. |
| Column Type | Precipitation, Filtration, n/a |
| Product Line | SDS-Out |
| Purification Target | Detergent Removal |
| Sufficient For | 200 mL Protein Solution |
| Quantity | 10 mL kit |
| Format | Kit, Microcentrifuge, Spin Column |
For Research Use Only. Not for use in diagnostic procedures.
Product Title
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