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Thermo Scientific™ Pierce™ Nickel Coated Plates

Thermo Scientific™ Pierce Nickel Coated Plates provide a simple format to bind His-tagged fusion proteins for ELISA and other plate-based assays involving protein interactions of expressed recombinant proteins.

297.00 € - 324.00 €

Specifications

Binding Property Standard Capacity, Affinity
Content And Storage Upon receipt store plates at 4°C in unopened pouches. Once opened, place unused plates in a resealable bag with desiccant and store at 4°C.
Packaging 5 Plates
Product Line Pierce
Product Type Microplate
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Products 4
Product Code Brand Description Detection Method Price Quantity & Availability  
Product Code Brand Description Detection Method Price Quantity & Availability  
10318783
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Thermo Scientific™
15142
Clear, 8-well Strip Colorimetric
309.00 €
Pack of 5
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Thermo Scientific™
15442
Clear, 96-well Colorimetric
297.00 €
Pack of 5
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Thermo Scientific™
15242
White, 96-well Chemiluminescence
324.00 €
Pack of 5
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Estimated Shipment: 02-08-2024
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Description

Description

These Ni(2+) chelate-coated plates are ideal for analyzing polyhistidine-tagged fusion proteins by ELISA-based methods. Proteins that contain a succession of several histidine residues at the amino or carboxyl terminus have a strong binding affinity for metal. Bacterial lysates containing polyhistidine-tagged fusion proteins can be added directly to the plates without the need for blocking. The clear, white or black plates can be used with colorimetric, chemiluminescent or fluorescent detection methods, respectively.

Thermo Scientific Pierce Nickel Coated White 96-Well Plates provide a simple format to bind His-tagged fusion proteins for ELISA and other plate-based assays involving protein interactions of expressed recombinant proteins. These white plates can be used with chemiluminescent detection methods.

Features of Pierce Nickel Coated Plates include:
• Ni2+ activated surface enables metal-chelate binding of His-tagged proteins
• Detergents used to lyse cells don't inhibit binding to activated plates as they do with plain polystyrene
• Better binding for sensitive assays compared to other commercially available nickel-activated plates
• Detection limit: 1 ng of polyhistidine fusion protein
• Binding capacity: approx. 9 pmol His-tagged protein (27 kDa) per well
• Activation level: 200 μL

These Ni(2+) chelate-coated plates are ideal for analyzing polyhistidine-tagged fusion proteins by ELISA-based methods. Proteins that contain a succession of several histidine residues at the amino or carboxyl terminus have a strong binding affinity for metal. Bacterial lysates containing polyhistidine-tagged fusion proteins can be added directly to the plates without the need for blocking.

Specifications

Specifications

Standard Capacity, Affinity
5 Plates
Microplate
200 μL
Divalent Nickel, Metal Chelate
Upon receipt store plates at 4°C in unopened pouches. Once opened, place unused plates in a resealable bag with desiccant and store at 4°C.
Pierce
Polystyrene
96
Blocker™ BSA Buffer
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